饲料研究2025，Vol.48Issue(1)：125-130,6.DOI:10.13557/j.cnki.issn1002-2813.2025.01.022
离子液辅助微波提取水藁本多糖工艺优化、分离纯化及抗氧化、抑菌活性研究
Study on optimization of ionic liquid-assisted microwave extraction of Ligusticum acuminatum Franch polysaccharide,separation and purification,antioxidant and antibacterial activities
摘要
Abstract
The experiment aimed to explore the optimal extraction process of Ligusticum acuminatum Franch(LAF)polysaccharides as well as their antioxidant and antibacterial activities.The extraction of LAF polysaccharides was conducted using an ionic liquid-assisted microwave extraction method.The optimal extraction process was determined through single-factor experiments and response surface optimization.After steps including defatting,decolorization,protein removal,dialysis,and purification,a uniform acidic polysaccharide(LAF-3)was obtained.The antioxidant and antibacterial effects were evaluated through in vitro tests.The results showed that when the microwave power is 300 W,the microwave time was 60 min,the liquid material ratio was 40 mL/g,the microwave temperature was 60 ℃,and the mass concentration of ionic liquid was 15 g/L,the highest extraction rate of LAF-3 was 41.481％.The IC50 of LAF-3 for scavenging 1,l-diphenyl-2-trinitrophenylhydrazine(DPPH)radical and total reducing capacity were 7.491 and 8.261 g/L.LAF-3 had a good inhibitory effect on Escherichia coli,Staphylococcus aureus and Candida albicans,the diameters of the inhibition zones were 12.13,11.35,and 10.08 mm respectively.The study indicates that the LAF-3 had good in vitro antioxidant and antibacterial ability.关键词
水藁本/多糖/提取工艺/抗氧化/抑菌Key words
Ligusticum acuminatum Franch/polysaccharide/extraction process/antioxidant/antibacterial分类
农业科技引用本文复制引用
张玲赫,李文文,谢浩,努尔买买提..离子液辅助微波提取水藁本多糖工艺优化、分离纯化及抗氧化、抑菌活性研究[J].饲料研究,2025,48(1):125-130,6.基金项目
2018年度植物生态学重点学科科研项目(项目编号:YLUPE201802) （项目编号:YLUPE201802）
2022年伊犁哈萨克自治州第二批科技计划项目(项目编号:YZ2022B040) （项目编号:YZ2022B040）
