饲料研究2025，Vol.48Issue(1)：137-142,6.DOI:10.13557/j.cnki.issn1002-2813.2025.01.024
植物乳植杆菌HYY-DB9冻干工艺优化及贮藏稳定性研究
Study on optimization of freeze-drying process and storage stability of Lactiplantibacillus plantarum HYY-DB9
摘要
Abstract
The aim of this experiment was to optimize the freeze-drying process of Lactiplantibacillus plantarum HYY-DB9.Using Lactiplantibacillus plantarum HYY-DB9 as the research subject,the study selected optimal centrifugation conditions,types and concentrations of freeze-drying protectants,and storage temperature and time.Through orthogonal experiments,a composite protectant formula was determined,and the storage stability of HYY-DB9 was verified.The results showed that when the centrifugation condition was set at 8 000 r/min for 10 minutes,the viable cell count of Lactiplantibacillus plantarum HYY-DB9 reached the maximum,with a survival rate of(92.39±5.84)％.The optimal composite protective agent formula was as follows:The addition amount of bacteriological peptone was 4％,the addition amount of trehalose was 8％,and the addition amount of sodium glutamate was 6％.Under this condition,the survival rate of Lactiplantibacillus plantarum HYY-DB9 reached(94.67±1.89)％,and the survival rate of Lactiplantibacillus plantarum HYY-DB9 stored at-20 ℃ for 28 days was(86.94±0.74)％.The study indicated that this protective agent formula had a good protective effect on the freeze-drying and storage process of Lactiplantibacillus plantarum HYY-DB9.关键词
植物乳植杆菌HYY-DB9/冷冻干燥/冻干保护剂/贮藏Key words
Lactiplantibacillus plantarum HYY-DB9/freeze-drying/lyophilized protectant/storage分类
农业科技引用本文复制引用
黄燕燕,黄佳蓉,黄芳,洪真真,孔小慧,汪浪红..植物乳植杆菌HYY-DB9冻干工艺优化及贮藏稳定性研究[J].饲料研究,2025,48(1):137-142,6.基金项目
国家自然科学基金(项目编号:32302242) （项目编号:32302242）
广东省基础与应用基础研究项目(项目编号:2022A1515110426) （项目编号:2022A1515110426）
广东省科技创新战略专项资金项目(项目编号:2022B1212010015) （项目编号:2022B1212010015）
