饲料研究2025，Vol.48Issue(1)：143-149,7.DOI:10.13557/j.cnki.issn1002-2813.2025.01.025
贝莱斯芽孢杆菌Y01的生物学特性及其对肉鸡生长性能的影响
Biological characteristics of Bacillus velezensis Y01 and its effects on growth performance of broilers
摘要
Abstract
The experiment aimed to explore the biological characteristics of Bacillus velezensis and its effects on the growth performance of broilers.Through bacterial separation and purification,morphological features,physiological and biochemical identification,and 16S rRNA sequence analysis,a strain of Bacillus velezensis Y01 was isolated and identified from the cecum of broilers.The drug sensitivity,enzyme production capacity,antibacterial activity,hemolytic property,and safety of this strain were also determined.A total of 120 one-day-old broilers(half male and half female)were randomly divided into two groups,with six replicates per group and 10 chickens per replicate.The control group was fed with mineral water mixed with feed,while the experimental group was fed with feed mixed with 1 x 108 CFU/mL Bacillus velezensis Y01 bacterial suspension.The experiment lasted for 25 days.The results showed that Bacillus velezensis Y01 was sensitive to 21 commonly used antibiotics,such as midecamycin,clindamycin,penicillin,norfloxacin,ampicillin,tobramycin,and erythromycin.It had the ability to produce protease and amylase,and showed good inhibitory effects on Staphylococcus aureus and Staphylococcus epidermidis,with no hemolytic phenomenon observed.Compared with control group,the final weight of broilers in experimental group was significantly increased(P＜0.05),and the ratio of feed to gain was significantly decreased(P＜0.05).The study indicates that Bacillus velezensis Y01 can inhibit Staphylococcus and is less likely to develop drug resistance.It can improve the growth performance of broilers and reduce the feed-to-weight ratio,providing experimental references for its use as a feed additive.关键词
贝莱斯芽孢杆菌/生物学特性/生长性能/益生菌Key words
Bacillus velezensis/biological characteristics/growth performance/probiotics分类
畜牧业引用本文复制引用
于鲁敏,王晓哲,徐立楠,张玲玲,曹晓燕,田亚晨,张津,张兴林..贝莱斯芽孢杆菌Y01的生物学特性及其对肉鸡生长性能的影响[J].饲料研究,2025,48(1):143-149,7.基金项目
国家自然科学基金(项目编号:32202810) （项目编号:32202810）
山东省自然科学基金(项目编号:ZR2022QC115) （项目编号:ZR2022QC115）
