饲料研究2025，Vol.48Issue(1)：157-162,6.DOI:10.13557/j.cnki.issn1002-2813.2025.01.027
红色红曲菌BM0321液态发酵产莫纳可林K发酵条件探究
Study on optimization of liquid fermentation process for Monacolin K production by Monascus ruber BM0321
邢宏博 1郑玉娇 2蔡汉聪 2宋敏 3卢秋咏 2邹新华 3曾运东 4黄明媛1
作者信息
- 1. 农业农村部饲料合成生物技术重点实验室,江西赣州 341000||广州播恩动物营养有限公司,广东 广州 510000
- 2. 农业农村部饲料合成生物技术重点实验室,江西赣州 341000
- 3. 农业农村部饲料合成生物技术重点实验室,江西赣州 341000||播恩集团股份有限公司,江西赣州 341000
- 4. 播恩集团股份有限公司,江西赣州 341000
- 折叠
摘要
Abstract
The experiment aimed to explore the fermentation conditions for the production of Monacolin K by the Monascus ruber BM0321 in liquid fermentation.The trial employed ambient temperature and pressure plasma mutagenesis technology to mutate the original strain,Monascus ruber BM0157,and selected a high-yielding and stable strain.Subsequently,single-factor experiments combined with response surface methodology were used to optimize the trial to determine the optimal fermentation conditions for Monascus ruber BM0321.The results showed that under the conditions of 145.42 g/L glycerol,261.6 mL/L soybean hydrolysate,80 g/L rice powder,2.1 g/L ZnSO4·7H2O,5 g/L corn steep powder,pH 5.5,10％inoculum size,and 20％loading volume,the Monacolin K content could reach 1 093.5 mg/L through shake flask fermentation.In a 100 L fermenter batch fermentation trial with Monascus ruber BM0321,the Monacolin K content could reach 848.23 mg/L under these conditions.The study indicates that the results can provide a reference for the industrial production of Monacolin K by liquid fermentation of Monascus ruber BM0321.关键词
红色红曲菌/液态发酵/莫纳可林K/常温常压等离子诱变/响应面法Key words
Monascus ruber/liquid fermentation/Monacolin K/room temperature and atmospheric pressure plasma mutagenesis/response surface methodology分类
农业科技引用本文复制引用
邢宏博,郑玉娇,蔡汉聪,宋敏,卢秋咏,邹新华,曾运东,黄明媛..红色红曲菌BM0321液态发酵产莫纳可林K发酵条件探究[J].饲料研究,2025,48(1):157-162,6.
