土壤与作物2025，Vol.14Issue(1)：95-107,13.DOI:10.11689/sc.2024052101
大豆根中响应早期共生固氮的转录组分析
Transcriptome analysis of soybean roots in response to early symbiotic nitrogen fixation
摘要
Abstract
In order to explore the key genes in response to early symbiotic nitrogen fixation in soybean roots as well as the functions and metabolic pathways of these key genes,this study used transcriptomics technology to perform RNA sequencing on soybean root materials one day after with or without inoculation of rhizobia.The key genes involved in the early symbiotic nitrogen fixation process were explored by GO enrichment and KEGG enrichment analysis of differentially expressed genes,and the transcription factors regulated by rhizobia were further screened.The AP2/ERF family members with the largest number of enrichments were subjected to evolutionary analysis.Finally,the expression of some genes was verified by qRT-PCR.A total of 824 differentially expressed genes(DEGs)in response to rhizobium infection are identified.GO enrichment and KEGG enrichment analysis showed that DEGs are mainly enriched in transcriptional regulation,hormone-related and secondary metabolite synthesis pathways.Phylogenetic analysis showed that AP2/ERF family members in soybean could be clustered into three branches according to their genetic relationship with MtERN1/2 and LjERN.Among them,branch Ⅰ may play a role in inducing rhizobium infection and nodule organo-genesis.The results of qRT-PCR showed that the AP2/ERF transcription factor is indeed regulated to varying degrees after inocula-tion with rhizobia.The DEGs found in this study provide candidate molecules for a better understanding of the early symbiotic nitrogen fixation mechanism.关键词
大豆/共生固氮/转录组/AP2/ERF转录因子Key words
soybean/symbiotic nitrogen fixation/transcriptome/AP2/ERF transcription factor分类
农业科技引用本文复制引用
黄慧超,王佳龙,于春静,潘钰,赵晓宇,周舒扬..大豆根中响应早期共生固氮的转录组分析[J].土壤与作物,2025,14(1):95-107,13.基金项目
黑龙江省科学院中试熟化项目(ZSSH2024SW01). （ZSSH2024SW01）
