武警医学2025，Vol.36Issue(2)：124-128,5.
吡非尼酮对大鼠坐骨神经损伤后神经再生和功能恢复的作用
Effects of pirfenidone on nerve regeneration and functional recovery after sciatic nerve injury in rats
摘要
Abstract
Objective To investigate the effect of pirfenidone on nerve regeneration in rats with sciatic nerve injury.Methods Rats with sciatic nerve injury were divided into four groups:normal saline group(control group,Ctrl),25 mg/kg pirfeni-done group(low-dose group,LG),50 mg/kg pirfenidone group(medium-dose group,MG),and 100 mg/kg pirfenidone group(high-dose group,HG).An autograft repair model was established.The morphology of regenerative nerves was evaluated by electron micros-copy(TEM).The recovery of motor function was assessed by Masson staining and sciatic functional index(SFI).The levels of trans-forming growth factor-β1(TGF-β1)in regenerative nerves were determined by immunohistochemical staining.Results TEM results showed that the diameter of myelinated nerve fibers and the mean thickness of myelin sheath in the MG and HG groups were higher than those in the Ctrl and LG groups,and the G ratio were lower than those in the Ctrl and LG groups at 12 weeks after surgery.At both 8 and 12 weeks after surgery,the value of the percentage of gastrocnemius fibers and SFI in the MG and HG groups were significantly higher than those in the Ctrl and LG groups,and the differences were statistically significant(P<0.05).Immunohistochemical results indicated that TGF-β1 expression in the MG and HG groups was significantly down-regulated compared with the Ctrl and LG groups,and the difference was statistically significant(P<0.05).Conclusions Pirfenidone can promote nerve regeneration and improve mus-cular function in rats with peripheral nerve injury,which may be related to its anti-fibrotic properties of pirfenidone.关键词
吡非尼酮/周围神经损伤/神经瘢痕/神经再生Key words
pirfenidone/peripheral nerve injury/nerve scarring/nerve regeneration分类
临床医学引用本文复制引用
漆景天,张永光,徐永君,盛青松,蔡龙宇,叶永平..吡非尼酮对大鼠坐骨神经损伤后神经再生和功能恢复的作用[J].武警医学,2025,36(2):124-128,5.基金项目
福建省自然科学基金(2021J011273,2022J01492) （2021J011273,2022J01492）
联勤保障部队第900医院战创伤救治课题(2023ZS07) （2023ZS07）
