中国病理生理杂志2025，Vol.41Issue(2)：322-330,9.DOI:10.3969/j.issn.1000-4718.2025.02.013
持续光照对小鼠肠道菌群的影响
Impact of continuous light exposure on gut microbiota of mice
摘要
Abstract
AIM:To observe the effect of continuous light on the structure and differential metabolites of gut microbiota in mice.METHODS:The mice were randomly divided into normal light(light/dark,LD)group and 24-hour continuous light(light/light,LL)group.The body weight,fasting blood glucose,serum free fatty acids,serum triacylglycerol and serum total cholesterol levels of each group of mice were measured after 10 weeks.Fresh feces were collected,and 16S rRNA sequencing technology was used to study the effect of continuous light on the diversity,structure,and species composition of gut microbiota in mice.Additionally,liquid chromatography-mass spectrometry(LC-MS)analysis was per-formed to observe the effect of continuous light on the metabolites in mice.RESULTS:Compared with the LD group,the body weight,fasting blood glucose and lipid levels of the LL group were increased(P＜0.05).At the phylum level,the proportion of Firmicutes in the LL group increased,while the proportion of Bacteroidetes decreased.At the class level,the abundance of norank_f_Muribaculaceae and Prevotellaceae_UCG-001 in the LL group decreased significantly,while the abundance of Lactobacillus,Turicibacter and Odoribacter increased significantly.Non-targeted metabolomics analysis iden-tified 65 and 73 differential metabolites under positive and negative modes,involving six major metabolic pathways,in-cluding ABC transporters,purine metabolism,pyrimidine metabolism,secondary bile acid biosynthesis,protein digestion and absorption,and choline metabolism in cancer.CONCLUSION:The structure and metabolites of gut microbiota in mice exposed to continuous light are relatively specific,and inosine may be a key biomarker and potential therapeutic tar-get for biological clock disorders.关键词
持续光照/生物钟/肠道菌群Key words
continuous light/circadian clock/gut microbiota分类
基础医学引用本文复制引用
李燕,郑路,汤茹,魏健,石佩,李战,王有琴..持续光照对小鼠肠道菌群的影响[J].中国病理生理杂志,2025,41(2):322-330,9.基金项目
山西省卫生健康委员会课题资助项目(No.2020140) （No.2020140）
长治医学院附属和平医院院级课题资助项目(No.HPYJ202018) （No.HPYJ202018）
