中国病理生理杂志2025，Vol.41Issue(2)：331-337,7.DOI:10.3969/j.issn.1000-4718.2025.02.014
枳连丸水提取物通过调控TLR4/MyD88/NF-κB信号通路减轻溃疡性结肠炎小鼠肠道损伤
Zhilianwan decoction attenuates intestinal injury in ulcerative colitis mice by modulating TLR4/MyD88/NF-κB signaling pathway
摘要
Abstract
AIM:This study aims to investigate the mechanism by which Zhilianwan(ZLW)decoction affects dextran sodium sulfate(DSS)-induced ulcerative colitis(UC)in mice,focusing on the Toll-like receptor 4(TLR4)/myeloid differentiation factor 88(MyD88)/nuclear factor-κB(NF-κB)signaling pathway.METHODS:Fifty male C57BL/6J mice were allowed ad libitum access to food for 1 week before the experiment.They were randomly divided into 5 groups:control group,model group,mesalazine(ME)group,high-dose ZLW(ZLW-H)group,and low-dose ZLW(ZLW-L)group,with ten mice in each group.All mice,except those in control group that received distilled water,were given a 3%DSS solution for seven consecutive days,starting the intervention on the first day of modeling.Each group re-ceived their respective treatments via gavage at volume of 20 μL/g for ten consecutive days.The mice in control and model groups received an equivalent volume of distilled water,while those in ME,ZLW-H,and ZLW-L groups were adminis-tered 0.2 g·kg-1·d-1 of ME,10.92 g·kg-1·d-1 of ZLW,and 2.73 g·kg-1·d-1 of ZLW,respectively.Body weight and dis-ease activity index(DAI)scores were recorded daily.At the end of the intervention,colon length was measured,and he-matoxylin-eosin staining was performed to assess pathological changes in colon tissues.ELISA and qPCR were employed to measure serum levels of interleukin-1β(IL-1β),IL-6,tumor necrosis factor-α(TNF-α)and IL-10,as well as the mRNA expression levels in colon tissues.Western blot analysis was conducted to evaluate the protein levels of TLR4,MyD88,cytosolic NF-κB and nuclear NF-κB in colon tissues.RESULTS:Compared with model group,the mice treated with ZLW exhibited an increase in body weight and colon length(P＜0.05),along with a decrease in DAI and colon histo-pathological scores(P＜0.05).Serum levels of IL-1β,IL-6,and TNF-α were significantly lower(P＜0.05),while IL-10 levels increased(P＜0.05).The mRNA expression levels of these inflammatory factors in colonic tissues reflected similar trends.Additionally,levels of TLR4,MyD88,and nuclear NF-κB proteins were reduced(P＜0.05),whereas cytosolic NF-κB protein expression was elevated(P＜0.05)in the colon tissues of treated mice.CONCLUSION:ZLW exerts at-tenuating effects on DSS-induced intestinal injury in UC mice,though modulation of the TLR4/MyD88/NF-κB signaling pathway.关键词
枳连丸/溃疡性结肠炎/炎症因子/TLR4/MyD88/NF-κB信号通路Key words
Zhilianwan/ulcerative colitis/inflammatory factors/TLR4/MyD88/NF-κB signaling pathway分类
基础医学引用本文复制引用
孙宇杰,胡嘉敏,马敏,曾华华,黄德斌..枳连丸水提取物通过调控TLR4/MyD88/NF-κB信号通路减轻溃疡性结肠炎小鼠肠道损伤[J].中国病理生理杂志,2025,41(2):331-337,7.基金项目
国家自然科学基金资助项目(No.82060745) （No.82060745）
风湿性疾病发生与干预湖北省重点实验室资助项目(No.PT022404) （No.PT022404）
湖北民族大学研究生科研创新项目(No.MYK2024078) （No.MYK2024078）
