中国草地学报2025，Vol.47Issue(2)：56-69,14.DOI:10.16742/j.zgcdxb.20240103
代谢组和转录组联合解析马蔺花瓣花青素积累机制
Metabolome and Transcriptome Combined Analysis of Anthocyanin Accumulation Mechanism in Iris lactea var.chinensis Accession Petals
摘要
Abstract
The flower color formation of Iris lactea var.chinensis is closely related to the anthocyanin content in petals.In order to analyze the accumulation process and gene regulation pathway in Iris lactea var.chinensis,this research focused on petals at squaring and full-bloom stages in violet and light blue germplasm.Ultra-high performance liquid chromatography-tandem mass spectrometry and high-throughput RNA sequencing methods were applied to analyze the transcriptome and targeted metabolome of anthocyanin synthesis in petals.Anthocyanin targeted metabolism analysis showed that 46 anthocyanin metabolites were identified in the petals of Iris lactea var.chinensis,in which 34 and 32 differential metabo-lites were detected in violet-light blue germplasm at budding stage and full flowering stage,respectively.Transcriptome analysis revealed 26774 and 25371 differentially expressed genes were screened out in violet-light blue at squaring stage and full-bloom stage,respectively.37 structural genes involved in antho-cyanin synthesis were identified,among which encoded 8 key enzymes.A total of 11 metabolites and 23 genes were screened by joint analysis.Correlation analysis indicated that the expression levels of CHS,CHI,F3H,DFR,3AT,BZ1 and other genes were significantly correlated with the accumulation of antho-cyanin metabolites.In summary,the vital metabolites for flower color difference of I.lactea are dihydromyric-etin,delphinidin,delphinidin-3-O-sophoroside,delphinidin-3-O-(6-O-malonyl-β-D-glucoside),delphinidin-3-O-sambubioside,delphinidin-3-O-glucoside,delphinidin-3-O-rutinoside,and malvidin-3-O-glucoside,whose synthesis and accumulation are regulated by F3´H,DFR,3AT,and BZ1 genes.关键词
马蔺/花色/代谢组/转录组/联合分析Key words
Iris lactea var.chinensis/Flower color/Metabolome/Transcriptome/Joint analysis分类
农业科技引用本文复制引用
李楠,毛培春,郑明利,云岚,田小霞,孟林..代谢组和转录组联合解析马蔺花瓣花青素积累机制[J].中国草地学报,2025,47(2):56-69,14.基金项目
北京市农林科学院科技创新能力建设专项(KJCX-20230109,KJCX20200107) （KJCX-20230109,KJCX20200107）
北京市自然科学基金项目(6142007) （6142007）
