医疗卫生装备2025，Vol.46Issue(2)：24-30,7.DOI:10.19745/j.1003-8868.2025024
八通道基因分析仪的设计
Design of 8-channel gene analyzer
马世宏 1钱宇 1梁松 1李夏彬 1张晶晶 1赵元 1王伟 1曹健荣1
作者信息
- 1. 德诺杰亿(北京)生物科技有限公司,北京 100176
- 折叠
摘要
Abstract
Objective To design an 8-channel gene analyzer to take the place of the widely used gene analyzer with problems in inconvenient consumable replacement and short storage time of electrophoresis polymer.Methods The 8-channel gene analyzer had its mechanical components composed of an automatic sample loading table,a polymer injection module,a high-voltage temperature control module,an optical module and an integrated U box,its electrical control system made up of a host computer(an embedded computer)and three slave computers(a sampling control board,a polymer injection control board and a high-voltage temperature control board).The automatic sample loading table involved in four motors and transmission systems for x,y,z directions and optical alignment,the transmission systems adopted mainly belt drive mode and the optical alignment motor had its threads with an anti-backlash structure;the polymer injuection module was manipulated by the polymer injection control board,and the polymer block was made of highly transparent acrylic material;the high-voltage temperature control module realized the regulation of electrophoresis voltage and the detection of electrophoresis current by the low-ripple precision high-voltage power supply,and controlled the temperature of the heating furnace by the proportional-integral-differential(PID)algorithm;the optical module consisted of an excitation module and a light-receiving module,which had the base of the reflector made of low expansion coefficient alloy material;the integrated U box had the electrophoresis polymer,capillary array,polymer block and anode buffer in a plastic housing;the host computer had the data acquisition software programmed with C# and C++,and the slave computers were controlled by STM32 SCM.Results The 8-channel gene analyzer had no significant differences with the widely used ABI3500 gene analyzer in resolution,precision accuracy and clinical results.Conclusion The 8-channel gene analyzer gains advantages in consumable replacement and storage time of electrophoresis polymer,and can meet the requirements for gene sequencing.[Chinese Medical Equipment Journal,2025,46(2):24-30]关键词
八通道/基因分析仪/基因测序/STM32单片机Key words
8 channel/gene analyzer/gene sequencing/STM32 SCM分类
医药卫生引用本文复制引用
马世宏,钱宇,梁松,李夏彬,张晶晶,赵元,王伟,曹健荣..八通道基因分析仪的设计[J].医疗卫生装备,2025,46(2):24-30,7.
