中国临床医学2025，Vol.32Issue(1)：93-100,8.DOI:10.12025/j.issn.1008-6358.2025.20241213
血液透析对外周组织中氧代二十碳四烯酸生物转化的影响
Effect of hemodialysis on the biotransformation of oxo-eicosatetraenoic acids in peripheral tissues
刘彤 1Maik Gollasch 2Friedrich C.Luft 3林攀 1吉俊 4孟尧4
作者信息
- 1. 复旦大学附属中山医院肾内科,上海 200032||上海市老年医学中心肾内科,上海 201100
- 2. 格赖夫斯瓦尔德大学医学院内科和老年病学系,格赖夫斯瓦尔德 17475
- 3. 夏里特医学院与马克斯-德尔布吕克分子医学中心联合机构,实验与临床研究中心,柏林 13125
- 4. 复旦大学附属中山医院肾内科,上海 200032
- 折叠
摘要
Abstract
Objective To analyze the differences of free and esterified oxo-eicosatetraenoic acids(oxo-ETEs)in blood cells and plasma from arterial and venous blood in hemodialysis(HD)patients.Methods Arterial and venous blood samples from 12 patients with end-stage renal disease(ESRD)before and after HD treatment at Charité-Universitätsmedizin Berlin,Germany,from June to December 2020 were collected.The esterified and free oxo-ETEs derived from arachidonic acid in blood cells and plasma were measured by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Results Neither esterified nor free oxo-ETEs in blood cells displayed significant arteriovenous differences before and after HD.HD predominantly affected the metabolic levels of esterified and free oxo-ETEs in plasma.HD reduced the arteriovenous differences of esterified 12-oxo-ETE,free 15-oxo-ETE,and free 5-oxo-ETE in plasma,while raised the arteriovenous differences of esterified 15-oxo-ETE.Conclusions The oxo-ETEs in blood cells are relatively well-stabilized responding to HD treatment,whereas arteriovenous differences of free and esterified oxo-ETEs in plasma are present and active in response to HD treatment,potentially contributing to the cardiovascular disease.关键词
血液透析/类二十烷酸/氧代二十碳四烯酸/生物转化/动静脉差异Key words
hemodialysis/eicosanoids/oxo-eicosatetraenoic acid/biotransformation/arteriovenous difference分类
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刘彤,Maik Gollasch,Friedrich C.Luft,林攀,吉俊,孟尧..血液透析对外周组织中氧代二十碳四烯酸生物转化的影响[J].中国临床医学,2025,32(1):93-100,8.
