中国蔬菜Issue(2)：71-81,11.DOI:10.19928/j.cnki.1000-6346.2024.5044
基于非靶向代谢组学的芦笋代谢物差异分析
Analysis of Different Metabolites in Asparagus by Non-targeted Metabolomics
摘要
Abstract
The metabolites in asparagus with different gender and color were determined and analyzed by extensive targeted metabolomics based on Ultra-high performance liquid chromatography-tandem mass spectrometry with'Lulusun No.7'as the material.The results showed that samples of GAF,GAM and WA were distinguished by PCA and OPLS-DA analysis.A total of 926 metabolites identified by detection were classified into 12 categories,among which flavonoids and phenolic acids were the most abundant,accounting for 35.6%of the total number of metabolites.Differential metabolites were screened and analyzed.It was found that contents of steroids in GAM were higher than that in GAF,and the contents of 31 out of 39 identified steroid metabolites were increased.The contents of steroidal saponins in GAM and GAF were lower than those in WA,and the number of down-regulated steroids accounted for about 84.6%of the total steroids.The contents of flavonoids and phenolic acids were higher than those in white asparagus,and the number of up-regulated accounted for about 62.4%and 45.5%of the total,respectively.Functional annotation and cluster analysis were carried out on the different metabolites in different gender and color asparagus,and 4 and 7 kinds of metabolites with larger difference multiple were found respectively.Diosgenin belonging to the steroid class was the unique differential metabolite of female and male asparagus.Coniferin,quercetin,3-phospho-D-glyceric acid,1-O-sinapyl-D-glucose and guanidinoacetate were unique differential metabolites of green and white asparagus.The results provide a theoretical basis for breeding,quality analysis and exploration of health functions in asparagus.关键词
芦笋/性别/颜色/代谢物Key words
asparagus/gender/color/metabolite引用本文复制引用
牛晓雪,包艳存,雷霆,李付军,李霞,牟萌,路远,李保华..基于非靶向代谢组学的芦笋代谢物差异分析[J].中国蔬菜,2025,(2):71-81,11.基金项目
潍坊市现代种业科研创新团队项目,山东省重点研发计划(农业良种工程)资助项目(2022LZGC024,2024LZGC032),潍坊市科技发展计划项目(2019ZJ1141),潍坊高新区科技惠民计划项目(2020KJHM14) （农业良种工程）
