中国蔬菜Issue(2)：123-130,8.DOI:10.19928/j.cnki.1000-6346.2025.0005
菊科蔬菜菌核病病原鉴定及其对氟唑菌酰羟胺的敏感性
Pathogen Isolation,Identification of Sclerotinina Disease on Asteraceae Vegetables and Its Sensitivity to Pydiflumetofen
李阿根 1朱颖 2程静雯 1石铖开 2张传清 2吴鉴艳2
作者信息
- 1. 杭州市余杭区农业生态与植物保护管理总站,浙江 杭州 311100||浙江农林大学现代农学院,浙江临安 311300
- 2. 浙江农林大学现代农学院,浙江临安 311300
- 折叠
摘要
Abstract
Sclerotinina disease is a devastating fungal disease with worldwide distribution known to affect many commercial crops.In this study,vegetables with typical symptoms of Sclerotinina were collected from Hangzhou City,Zhejiang Province to obtain the pathogen by tissue isolation.The pathogenic fungus was identified based on the morphologies and molecular biological analysis,and then the pathogenicity was tested sensitivity to pydiflumetofen was determined by mycelial growth rate test.The results showed that 62 Sclerotinia,isolates were obtained from diseased leaf lettuce and stem lettuce,and combined with ITS and laccase 2 gene sequence analysis,6 isolates were classified into Sclerotinia minor,other 56 isolates were S.sclerotiorum.On PDA medium,the mycelial growth rate of S.minor was significantly lower than that of S.sclerotiorum,but the time of sclerotiorum production of S.minor was earlier,and the number of sclerotiorum of S.minor was higher.The virulence of Sclerotinia isolates on different vegetables were confirmed,the isolates caused the typical watery and brown lesions,and form white mycelia and sclerotiorum on infected tissues at the later stage.Sclerotiorum in Asteraceae vegetables caused by S.minor was first reported in China,and comparing the lesion diameter caused by S.minor and S.sclerotiorum,the greater virulence of S.sclerotiorum was observed on all tested hosts.Pydiflumetofen sensitivity of Sclerotinia isolates was determined by the 50%effective concentration value,the results showed that 83.9%of the assayed isolates having an EC50 of less than 0.01 μg·mL-1.Our results suggested that pydiflumetofen and its mixtures could be selected for controlling Sclerotinina on Asteraceae vegetables.关键词
菊科蔬菜/核盘菌/小核盘菌/致病性/氟唑菌酰羟胺/敏感性Key words
Asteraceae vegetables/Sclerotinia sclerotiorum/Sclerotinia minor/pathogenicity/pydiflumetofen/sensitivity引用本文复制引用
李阿根,朱颖,程静雯,石铖开,张传清,吴鉴艳..菊科蔬菜菌核病病原鉴定及其对氟唑菌酰羟胺的敏感性[J].中国蔬菜,2025,(2):123-130,8.
