中国农业大学学报2025，Vol.30Issue(3)：13-23,11.DOI:10.11841/j.issn.1007-4333.2025.03.02
牛PPP1R3基因家族的生物信息学分析及PPP1R3C基因的mRNA表达水平分析
The bioinformatics analysis of the PPP1R3 gene family in cattle and the analysis of mRNA expression levels of the PPP1R3C gene
摘要
Abstract
To systematically analyze the evolutionary relationships,molecular structures,and functional characteristics of the PPP1R3C gene family across bovine and other species,this study employed bioinformatics approaches to investigate the genetic features of the PPP1R3 gene family,to which PPP1R3C belongs,and examined PPP1R3C expression levels using RT-qPCR.The results revealed:1)Members of the PPP1R3 gene family exhibited significant variations in their isoelectric points and molecular weights.2)Phylogenetic tree and conserved domain analyses demonstrated that PPP1R3 family members have evolved distinct motif and domain combinations throughout their evolution;synteny analysis revealed patterns of positional changes of PPP1R3 family members across the chromosomes of cattle,mice,humans,sheep,and pigs.3)RT-qPCR analysis demonstrated that PPP1R3C exhibits significantly high expression in bovine adipose and lung tissues,while showing minimal expression in muscle-related tissues;furthermore,its expression levels progressively increased during bovine adipocyte differentiation and maturation.In conclusion,this study investigated the evolutionary relationships,molecular structures,and functional characteristics of the PPP1R3 gene family across bovine and other species,highlighting the crucial regulatory role of PPP1R3C in lipid metabolism and energy homeostasis.These findings provide a theoretical foundation for further investigation of the specific mechanisms of this gene family under various physiological and pathological conditions.关键词
PPP1R3C/PPP1R3基因家族/表达分析/生物信息学分析Key words
PPP1R3C/PPP1R3 gene family/expression analysis/bioinformatics analysis分类
农业科技引用本文复制引用
吴昊,张久盘,宋雅萍,姜超,宋小雨,焦若普,杨东梅,马云,魏大为..牛PPP1R3基因家族的生物信息学分析及PPP1R3C基因的mRNA表达水平分析[J].中国农业大学学报,2025,30(3):13-23,11.基金项目
中央引导地方科技发展专项(2024FRD05052) （2024FRD05052）
国家自然科学基金项目(32060744,32202641) （32060744,32202641）
中科院"西部之光"人才培养计划项目(2024) （2024）
国家科技重大专项-科技创新2030专项(2023ZD0404803-02) （2023ZD0404803-02）
宁夏重点研发计划项目(2023BCF01006) （2023BCF01006）
