中国蔬菜Issue(2)：140-147,8.DOI:10.19928/j.cnki.1000-6346.2025.4002
广西生姜青枯病菌的遗传多样性与致病力分化
Genetic Diversity and Pathogenicity Differentiation of Ralstonia solanacearum Isolated from Ginger in Guangxi
摘要
Abstract
Ginger bacterial wilt is caused by Ralstonia solanacearum,which is an important soil-borne disease in the ginger production area of Guangxi.In order to clarify the genetic diversity and pathogenicity differentiation of R.solanacearum on ginger in Guangxi,this study collected strains,and conducted phylogenetic analysis based on the phylotype classification framework and egl gene sequence of the pathogen.At the same time,R.solanacearum strains were inoculated by irrigating the bacterial suspension after stabbing the stem base of ginger plant,and cluster analysis was conducted on the disease index.The results showed that all 42 strains of R.solanacearum isolated from main ginger production areas of Guangxi were identified as phylotype Ⅰ,belonging to sequevar 14,16 and 30,with the former two being the main types.Through pathogenicity testing,the strains were divided into three categories:64.29%of the strains showed very strong pathogenicity,with the disease index of 83.70 to 100.00;21.43%of the strains showed strong pathogenicity,with the disease index of 62.22 to 75.56;Only 14.29%of the strains showed weak to moderate pathogenicity,with the disease index of 24.44 to 51.11.These results indicated that there is a certain degree of genetic diversity and significant pathogenicity differentiation in the population of R.solanacearum on ginger in Guangxi,providing important references for researching their pathogenic mechanisms and breeding for resistance to the disease.关键词
生姜/青枯雷尔氏菌/遗传多样性/致病力分化Key words
ginger/Ralstonia solanacearum/genetic diversity/pathogenicity differentiation引用本文复制引用
杨维超,李小莉,黄皓,陈媛媛,文俊丽,周生茂,袁高庆..广西生姜青枯病菌的遗传多样性与致病力分化[J].中国蔬菜,2025,(2):140-147,8.基金项目
广西重点研发计划项目(桂科AB21238002),广西农业科学院基本科研业务专项(2015YZ03,桂农科2024YP082) （桂科AB21238002）
