中国农业大学学报2025，Vol.30Issue(3)：37-48,12.DOI:10.11841/j.issn.1007-4333.2025.03.04
circFAM53B在奶牛乳腺上皮细胞炎症反应中的作用
Role of circFAM53B in the inflammatory response of bovine mammary epithelial cells
摘要
Abstract
To investigate the function of circFAM53B in dairy cow mastitis,this study took bovine mammary epithelial cells(bMECs)as the research object,and bioinformatics was used to predict the function of circFAM53B.The effects of circFAM53B on cell inflammation,proliferation and apoptosis were examined by interfering or overexpressing circFAM53B in LPS-induced bMECs inflammation.The results showed that:1)Dairy cow circFAM53B was a real-existing circular RNA,mainly located in the cytoplasm of bMECs.2)The expression level of dairy cow circFAM53B was highly significantly up-regulated in LPS-induced inflammatory response of bMECs(P＜0.01).3)In the inflammatory response of bMECs,circFAM53B in cows can promote the expression and secretion of inflammatory factors,inhibits cell viability and proliferation,and promote apoptosis in bMECs inflammation,and exacerbate the development of inflammation.In conclusion,circFAM53B may be involved in the regulation of cow mastitis and promote the inflammatory process.This study lays a foundation for molecular breeding for resistance to cow mastitis.关键词
环状circRNA/奶牛/乳房炎/乳腺上皮细胞/大肠杆菌Key words
circular RNA/dairy cow/mastitis/mammary epithelial cells/E.coli分类
畜牧业引用本文复制引用
周冉,王兴平,虎喜敏,李宇航,王正兴,罗仍卓么..circFAM53B在奶牛乳腺上皮细胞炎症反应中的作用[J].中国农业大学学报,2025,30(3):37-48,12.基金项目
国家自然科学基金项目(32060749) （32060749）
宁夏自然科学基金项目(2023AAC03049) （2023AAC03049）
国家现代农业产业技术体系项目(CARS-36) （CARS-36）
