中国农业大学学报2025，Vol.30Issue(3)：85-94,10.DOI:10.11841/j.issn.1007-4333.2025.03.08
秸秆基质块育秧过程中酚酸释放规律及自毒作用消减
Release mechanism of phenolic acids and autotoxicity reduction in the process of seedling raising with straw substrate block
摘要
Abstract
The purpose of this study was to analyze the release pattern of phenolic acids and their biological toxicity to rice in the process of seedling raising with straw substrate block,and to explore the feasibility of manganese oxide for reducing phenolic acids and improving seedling raising effect.Experiments on seedling raising were conducted using straw substrate block and rice"Nanjing 46"as research objects.The main types and contents of phenolic acids released in the process of seedling raising with straw substrate block were determined by using liquid chromatography-mass spectrometry to measure the dynamic changes of phenolic acid compounds.The biological toxicity of main phenolic acids was determined by germination test.Furthermore,manganese dioxide was selected as an additive to detect its removal effect on phenolic acids and the improvement effect on seedling raising with straw substrate block.The results showed that:1)During the 15 d seedling raising period,the concentration of total phenolic acids produced by straw substrate block increased first and then decreased,and the highest concentration was 328 mg/L on the 10th day.The content of p-coumaric acid was the highest in the emergence stage(0 to 3 d),followed by ferulic acid,which accounted for about 95％of the main phenolic acids;2)When the concentration of p-coumaric acid reached 200 mg/L and ferulic acid reached 350 mg/L,the germination index of rice seeds decreased significantly;3)The degradation rates of p-coumaric acid and ferulic acid by manganese dioxide were negatively correlated with the phenolic acid concentration.The degradation rates were 49.9％and 69.9％at 100 mg/L,25.8％and 32.2％at 300 mg/L,respectively;4)The addition of manganese dioxide in straw substrate block could effectively improve the effect of rice seedling raising,and the plant height,stem diameter,dry weight of aboveground and underground parts were significantly increased.When the additional amount was 0.025％,the effect of seedling raising was the same as commercial substrate.In conclusion,the main phenolic acids released in the early stage of straw substrate seedling were p-coumaric acid and ferulic acid.Manganese dioxide had a good degradation effect on them.Adding manganese dioxide to the straw substrate block could significantly improve the rice seedling-raising effect.关键词
秸秆基质块/育秧/酚酸/自毒作用/二氧化锰Key words
straw substrate block/seedling raising/phenolic acid/autotoxicity/manganese dioxide分类
农业科学引用本文复制引用
张晶,曲萍,赵奕然,孙恩惠,雍宬,曹云,孙倩,靳红梅,黄红英..秸秆基质块育秧过程中酚酸释放规律及自毒作用消减[J].中国农业大学学报,2025,30(3):85-94,10.基金项目
江苏省农业科技自主创新项目(CX(23)1034) （CX(23）
农业农村部种养结合重点实验室开放课题(202301) （202301）
江苏省农科院资环所2022年度青年联合创新项目(ZCX(2022)22) （ZCX(2022）
江苏农垦科技创新专项(SKNF012024003) （SKNF012024003）
