中国农业大学学报2025，Vol.30Issue(3)：141-152,12.DOI:10.11841/j.issn.1007-4333.2025.03.13
mTOR通路相关基因在不同品系鸡不同组织、细胞的表达规律及相关性分析
Expression pattern and correlation analysis of mTOR pathway related genes in different tissues and cells of different strains of chickens
摘要
Abstract
In order to investigate the regulatory role of GRB10,ULK2 and PIK3R1 genes enriched in the mTOR pathway in chicken fat deposition and muscle development,the present study was conducted to analyse the differences in slaughter performance,pectoral muscle and serum levels of triglycerides(TG),phospholipids(PL),and cholesterol(CHO)using the S3,F,and H lines,and then to detect the expression changes of the three genes using fluorescence qPCR in expression changes in different tissues and cells,and do correlation analysis.The results showed that:1)The F line had the significantly highest(P＜0.05)in body weight,full net chamber weight,pectoral muscle weight,leg muscle weight and abdominal fat weight,and the H line had the significantly lowest(P＜0.05)in abdominal fat rate.2)Lipid contents in pectoral muscle and serum showed a wave pattern of change in different developmental periods.The TG content in the pectoral muscle was significantly(P＜0.05)highest in the S3 line at 2 and 15 weeks of age,and CHO was significantly(P＜0.05)lowest in the H line at 2 and 5 weeks of age;the TG content in the serum was significantly(P＜0.05)highest in the F line at 5 weeks of age,and the LDL-C was significantly(P＜0.05)highest in the F line at 2,5,and 15 weeks of age.3)The diameter/density of muscle fibres in the H line was significantly lower/higher than that of the other lines.density was significantly lower/higher than that of other lines(P＜0.05),while the cross-sectional area of myofibrils was the opposite,with adipocytes in line S3 having the significantly largest volume(P＜0.05).4)The three genes were expressed in liver,abdominal lipids,pectoral muscles,leg muscles,sebaceous tissues,adult and intramuscular myofibroblasts in chickens of different lines at different weekly ages,showing significant line and tissue variability(P＜0.05).5)GRB10,ULK2 and PIK3R1 expression showed significant positive correlation with pectoral muscle weight(rate)and leg muscle weight(rate)(P＜0.05),and ULK2 was significantly positively correlated with abdominal fat weight(rate)(P＜0.05).In conclusion,the lipid levels in breast muscle and serum fluctuated significantly during growth and development of different breeds of chickens;the three genes were abundantly expressed in multiple tissues with significant differences among breeds and tissues,and were significantly correlated with breast muscle weight(rate),leg muscle weight(rate),and abdominal fat weight(rate),making them potential targets for the study of muscle development and lipid deposition in chickens.This study provides a solid theoretical foundation and data support for the in-depth study of the functions and mechanisms of GRB10,ULK2 and PIK3R1.关键词
鸡/CGRB10/ULK2/PIK3R1/肌肉发育/脂肪沉积Key words
chicken/GRB10/ULK2/PIK3R1/muscle development/fat deposition分类
农业科技引用本文复制引用
黄华云,吴香君,梁忠,杨苗苗,向海,李瑞瑞,赵振华,李春苗,王钱保,黄正洋,张耿铭..mTOR通路相关基因在不同品系鸡不同组织、细胞的表达规律及相关性分析[J].中国农业大学学报,2025,30(3):141-152,12.基金项目
"国家重点研发计划农业生物种质资源挖掘与创新利用"重点专项资助(2021YFD1200803) （2021YFD1200803）
现代农业产业技术体系建设专项(CARS-41-Z05) （CARS-41-Z05）
江苏省种业振兴揭榜挂帅项目((JBGS(2021)109 （(JBGS(2021）
JBGS(2021)029)) （2021）
