中国油料作物学报2025，Vol.47Issue(1)：45-59,15.DOI:10.19802/j.issn.1007-9084.2023278
甘蓝型油菜BnaC3.CCD4转基因白花株系白天花色变化的转录组分析
Transcriptomic analysis on the flower color changes of white-petaled BnaC3.CCD4-transgenic rapeseed(Brassica napus)lines during the daytime
摘要
Abstract
Our previous study revealed that BnaC3.CCD4 encodes carotenoid cleavage dioxygenas whose muta-tions altered flower color from white to yellow in rapeseed.Interestingly,we observed that the white flowers of func-tional BnaC3.CCD4 transgenic lines showed pale yellow in the morning and still became pure white in the afternoon during the daytime.In order to explore whether environmental signals such as light and temperature have a regulato-ry effect on carotenoid biosynthesis and metabolism,RNA-seq was performed for transcriptomic analysis on them at two different timepoints in the present research.The analysis results of gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG)revealed that a number of differential expression genes were significantly enriched in pathways related to light signal,circadian rhythm,stimulus response and carotenoid biosynthesis et al.Through fur-ther analyzing the top 50 differentially expressed genes,it was revealed that the light signal might be the key factor for the flower color change of BnaC3.CCD4 transgenic lines.Therefore,it was inferred that the light signal factors potentially regulate the expressions of the genes in the carotenoid biosynthesis pathway by binding to their photores-ponsive elements to control the carotenoid contents in the petals and then cause their color change.关键词
甘蓝型油菜/花色/类胡萝卜素裂解双加氧酶/光信号/昼夜节律Key words
Brassica napus/flower color/carotenoid cleavage dioxygenas/light signal/circadian rhythm分类
农业科学引用本文复制引用
王锦婷,孙雪雨,胡文竞,杨谦,刘超..甘蓝型油菜BnaC3.CCD4转基因白花株系白天花色变化的转录组分析[J].中国油料作物学报,2025,47(1):45-59,15.基金项目
武汉市生物育种关键技术攻关及新品种培育科技重大专项(2022021302024851) （2022021302024851）
