中国油料作物学报2025，Vol.47Issue(1)：60-70,11.DOI:10.19802/j.issn.1007-9084.2023263
延伸因子Elongator Complex Protein 2抗油菜菌核病的功能研究
Function of elongator complex protein 2 against Sclerotinia sclerotiorum of rapeseed
摘要
Abstract
Rapeseed production is severely restricted by Sclerotinia disease.Elongator complex protein(ELP)is a 6-subunit complex that interacts with RNA polymerase Ⅱ,involved in plant defense response.To deeply under-stand its gene function,we constructed vectors on overexpressed-BnA06.ELP2 and knockout-BnA06.ELP2(by gene editing)using homologous recombination method,and then investigated their transgenic plants.Disease resis-tance experiments showed that BnA06.ELP2 overexpression plants exhibited better resistance compared to wild-type,while gene edited BnA06.ELP2 plants led to a decrease of resistance.Further RT-qPCR analysis indicated that BnA06.ELP2 could regulate transcription of catalase BnCAT1 and BnCAT2(marker genes of JA/ET pathway),jasmonate synthesis related genes BnLOX2 and BnOPR1,and BnPDF1.These results indicated that BnA06.ELP2 function in rapeseed is involved in defense response against Sclerotinia sclerotiorum infection.关键词
甘蓝型油菜/延伸因子复合体/菌核病Key words
Brassica napus/elongator complex protein/Sclerotinia stem rot分类
农业科学引用本文复制引用
何督,张岩,罗倩,傅玉全,杜雪竹..延伸因子Elongator Complex Protein 2抗油菜菌核病的功能研究[J].中国油料作物学报,2025,47(1):60-70,11.基金项目
科技创新2030——重大项目(2023ZD04070) （2023ZD04070）
武汉市生物育种与新品种选育关键技术科技重大专项(2022021302024851) （2022021302024851）
农业农村部油料作物生物学与遗传育种重点实验室开放课题基金(KF2022001) （KF2022001）
