中国油料作物学报2025，Vol.47Issue(1)：94-104,11.DOI:10.19802/j.issn.1007-9084.2023050
二倍体野生种花生Oleosin基因家族全基因组鉴定及其表达分析
Genome-wide analysis and stress-responsive expression profiling of the Oleosin gene family in diploid wild species Arachis duranensis and Arachis ipaensis
摘要
Abstract
To identify the members of Oleosin gene family and analyze its structure and function,Hiddne Mar-kov Model(HMM)was used to search the protein databases of wild peanut(Arachis duranensis)and(Arachis ipaensis).The bioinformatic method was carried out to predict and analyse the physicochemical properties,gene structure,and gene location.The expression patterns of these genes in different peanut tissues and organs and under different stress conditions were analyzed by qRT-PCR.In this study a total of 7 AdOleosin genes and 6 AiOleosin genes were identified.Results showed that all Oleosin genes except Aiole-2 are acidic proteins.Further analysis showed that Oleosins were basic small molecular proteins with α-spiral as the main structure.They have 2-3 trans-membrane domains,a conserved central hydrophobic region and a highly conserved proline junction.The AdOleo-sin and AiOleosin gene families have similar chromosomal localization,which no localization on chromosomes 1 and 6,and 3 family members each on chromosomes 4 and 7.There are multiple hormone and stress-related cis-acting elements,indicating that the gene family is involved in stress response.Further,the expression patterns in different tissues were investigated by qRT-PCR.Results showed that the Oleosin gene expressed in peanut seeds is tempera-ture dependent and expression in peanut roots,stems,leaves were induced by drought stress,salt and ABA.The above results provide a theoretical basis for in-depth study of the gene function of the Oleosin family.关键词
花生/Oleosin基因家族/生物信息学/表达分析Key words
peanut/Oleosin gene family/bioinformatics/expression analysis分类
农业科学引用本文复制引用
姜焕焕,温思慧,卢雨庭,陈刚,王通..二倍体野生种花生Oleosin基因家族全基因组鉴定及其表达分析[J].中国油料作物学报,2025,47(1):94-104,11.基金项目
肇庆市科技创新指导项目(2022040316001) （2022040316001）
肇庆市科技局项目(2020N12003) （2020N12003）
肇庆学院校级项目(190060,QN202329) （190060,QN202329）
大学生创新创业训练计划项目(S202310580047,X202210580113) （S202310580047,X202210580113）
