中国药科大学学报2025，Vol.56Issue(1)：56-64,9.DOI:10.11665/j.issn.1000-5048.2024052201
叶酸有关物质的液相色谱-质谱联用分离与鉴定
Separation and identification of folic acid and its related substances by liquid chromatography-mass spectrometry
宁曼如 1徐柯卉 1杭太俊 1宋敏1
作者信息
- 1. 中国药科大学药物分析系,南京 210009
- 折叠
摘要
Abstract
The related substances in folic acid were separated on an ODS column by linear gradient elution using the mixture of 10 mmol/L ammonium acetate buffer solution and methanol(pH 6.3)as the mobile phase,and their accurate mass-charge ratio and elemental composition of parent and product ions were detected by electrospray ionization quadrupole time-of-flight mass spectrometry(ESI-Q-TOF/MS).The structures of the related substances were then determined by MS spectra elucidation,organic reaction mechanism analysis,or reference substances confirmation.Under the established LC-ESI-Q-TOF/MS conditions,folic acid and its related substances were adequately separated.Among the 23 main related substances found,2 were known impurities listed in European Pharmacopoeia,4 were consistent with the literature reports,and the remaining 17 were newly identified unknown related substances in this study.The results of separation and identification can provide some useful reference for the quality control of folic acid.关键词
叶酸/有关物质/降解产物/结构鉴定/液相色谱-质谱联用Key words
folic acid/related substances/degradation products/structural identification/LC-MS分类
药学引用本文复制引用
宁曼如,徐柯卉,杭太俊,宋敏..叶酸有关物质的液相色谱-质谱联用分离与鉴定[J].中国药科大学学报,2025,56(1):56-64,9.
