中国油料作物学报2025,Vol.47Issue(1):115-125,11.DOI:10.19802/j.issn.1007-9084.2023235
不同基因型向日葵芽期和苗期耐盐性分析
Analysis of salt-tolerance in different genotypes of sunflower at germination and seedlings
摘要
Abstract
To establish a simple and efficient method of salt tolerance assessment in sunflower,clarify the dif-ference in salt tolerance among different genotypes of sunflower and select the highly salt-tolerant cultivars,in this study,the seeds and seedlings from 20 sunflower varieties were subjected to different concentrations of NaCl salt so-lutions,then the changes of every index for each variety were observed under different treatments and time points,and correlation,principal component,membership function value,cluster analysis and comprehensive evaluation of salt tolerance were conducted based on each index to compare the salt tolerance of those varieties.The results showed that seed germination and seedling height of some varieties had a certain promotion effect under the treat-ments with the NaCl concentration below 160 mmol/L compared to the control.However,with the increase of con-centration and the delay of treatment time,seed germination and seedling growth were inhibited to different degrees and the suppression was more obvious.Under NaCl treatment,K+/Na+of seedling leaves was significantly higher than that of root,and the relative SPAD value initially increased and then decreased with the increase of NaCl con-centration.Under the same stress concentration,the relative values of each observed index of salt-tolerant varieties(compared with 0 mmol/L control)exhibited higher than those of salt-sensitive varieties,indicating that salt-toler-ant varieties were less affected by NaCl compare to salt-sensitive ones,and had a certain salt tolerance.It was con-cluded that the optimal concentration for NaCl salt tolerance identification in both sunflower germination and seed-ling stages was 250 mmol/L.The salt tolerance at the germination stage was compared by investigating the compre-hensive membership function values of relative germination rate,relative germination potential,relative radicle length and relative germ length;Salt tolerance of seedlings was determined on the 14 day after NaCl treatment(250 mmol/L),by examining the comprehensive membership function values of relative survival rate,relative leaf area,relative plant height,relative SPAD value,relative fresh weight of above-ground tissues and relative fresh weight of roots.Two varieties No.67 and HZ001 with salt tolerance were obtained by the above method.关键词
向日葵/耐盐性鉴定/芽期/苗期/聚类分析Key words
sunflower/identification of salt tolerance/germination stage/seedling stage/cluster analysis分类
农业科技引用本文复制引用
李玉骁,谭美莲,汪磊,朱梓榕,严兴初,汪魏,王玲..不同基因型向日葵芽期和苗期耐盐性分析[J].中国油料作物学报,2025,47(1):115-125,11.基金项目
向日葵种质资源精准鉴定项目(19221985) (19221985)
国家特色油料产业技术体系(CARS-14-1-17) (CARS-14-1-17)
油料作物优异种质资源发掘鉴定与创新利用(CAAS-OCRI-ZDRW-202203) (CAAS-OCRI-ZDRW-202203)