中国药科大学学报2025，Vol.56Issue(1)：73-79,7.DOI:10.11665/j.issn.1000-5048.2024050801
溃疡性结肠炎小鼠结肠及免疫组织中四逆散药源性成分的LC-MS分析
Analysis of xenobiotics in colon and immune tissues of ulcerative colitis mice after administration of Sini San by LC-MS
摘要
Abstract
Dysregulation of immune response is currently recognized as one of the important pathological factors in ulcerative colitis(UC).Based on the confirmation that the Sini San(SNS)can significantly improve the colon inflammation induced by dextran sulfate sodium sulfate(DSS)in mice,the present work systematically studied the xenobiotics in the colon and mesenteric lymph nodes,spleen,and thymus of UC mice after administration of SNS by high-performance liquid chromatography-ion trap time-of-flight mass spectrometry(HPLC-IT-TOF-MS).The results showed that,in addition to the colon,some components and their metabolites in SNS could be distributed in immune tissues,and it was found that the quality of relatively low-abundance and weakly responsive components such as saikosaponin a,paeoniflorin,and glycyrrhizic acid had the characteristics of efficient transmission to the colon and lymphoid organs.These components were very likely to be the source of pharmacodynamic substances of SNS.The findings of this study lay a foundation for the study of the efficacy and molecular mechanism of the components against ulcerative colitis,and also provide a scientific basis for the rational clinical application of SNS,which is expected to promote the secondary development of its preparations.关键词
四逆散/溃疡性结肠炎/结肠/免疫组织/药源性成分Key words
Sini San/ulcerative colitis/colon/immune tissues/drug-derived components分类
药学引用本文复制引用
曹艳芳,汪雅莉,王安慧,陈永顺,李思翰,冯凯,杨婉薇,宋瑞..溃疡性结肠炎小鼠结肠及免疫组织中四逆散药源性成分的LC-MS分析[J].中国药科大学学报,2025,56(1):73-79,7.基金项目
江苏省自然科学基金项目(BK20161456) （BK20161456）
商丘医学高等专科学校开放课题(KFKT23002) This study was supported by the Natural Science Foundation of Jiangsu Province(BK20161456),and the Research Grant from Shangqiu Medical College(KFKT23002) （KFKT23002）
