中国输血杂志2025，Vol.38Issue(2)：172-180,9.DOI:10.13303/j.cjbt.issn.1004-549x.2025.02.003
丹参注射液对HBOC-CHP01复苏失血性休克大鼠肾脏保护作用的研究
The renoprotective effect of Salvia miltiorrhiza injection on HBOC-CHP01 resuscitated haemorrhagic shock rats
摘要
Abstract
Objective To investigate the protective effect of Salvia miltiorrhiza injection(SMI)on the kidneys of HBOC-CHP01 resuscitated haemorrhagic shock rats.Methods A 50%haemorrhagic shock rat model was established,with 12 rats divided into two groups:SMI+HBOC-CHP01 group and HBOC-CHP01 group,with 6 rats in each group.The rats in the SMI+HBOC-CHP01 group were given an equal volume of HBOC-CHP01 for resuscitation after haemorrhagic shock,and an 8 mL/kg dose of SMI.Rats in the HBOC-CHP01 group were resuscitated by administering an equilibrium blood loss vol-ume of HBOC-CHP01 and given an 8 mL/kg dose of 0.9%NaCl solution.Blood was taken from rats at five points:before bloodletting(baseline),during haemorrhagic shock(HS),immediately after resuscitation(RS0h),1 h after resuscitation(RS1h),and 24 h after resuscitation(RS24h).A blood gas analyser was used to detect the lactate level(Lac),glucose content(Glu),residual base(BEecf),pH,bicarbonate(HCO3-),high iron haemoglobin(MetHb).White blood cells(WBC),platelets(PLT),haemoglobin content(Hb),carboxyhaemoglobin(COHb)were detected using a quintuple classification.Blood creatinine(SCr),uric acid(UA),kidney-related indexes were detected using biochemistry instru-ment.Kidney tissues of the rats were taken after 24 h of resuscitation and after execution,and the inflammation of kidneys of the rats of the two groups was analyzed using HE staining.Fluorescence staining was used to detect the level of ROS in the kidneys of rats in both groups.Results At RS 0h,the Beecf,Glu and Lac levels of rats in the SMI+HBOC-CHP01 group were significantly lower than those of rats in the HBOC-CHP01 group,and the pH level of rats in the SMI+HBOC-CHP01 group was significantly higher than that of rats in the HBOC-CHP01 group,and the Glu levels of rats in the SMI+HBOC-CHP01 group were significantly lower than those of rats in the HBOC-CHP01 group at RS 1h.At RS 0h,the WBC,PLT and COHb contents of rats in the SMI+HBOC-CHP01 group were all significantly higher than those of rats in the HBOC-CHP01 group,and at RS 1h,the WBC content of rats in the SMI+HBOC-CHP01 group was significantly higher than that of rats in the HBOC-CHP01 group;at RS 1h,the UA content of rats in the SMI+HBOC-CHP01 group was significantly lower than that of rats in the HBOC-CHP01 group;at RS 24h,the SCr content of rats in the SMI+HBOC-CHP01 group was significantly lower than that of rats in the HBOC-CHP01 group;at RS 24h,the inflammation level of kidney tissues of rats in the SMI+HBOC-CHP01 group was significantly lower than that of rats in the HBOC-CHP01 group rats,and the ROS and MPO levels in the kidney tissues of rats in the SMI+HBOC-CHP01 group were significantly lower than those of rats in the HBOC-CHP01 group.Conclusion The combination of Salvia miltiorrhiza injection during the resuscitation of rats with se-vere haemorrhagic shock by HBOC-CHP01 can alleviate renal injury by reducing inflammatory response and oxidative stress.关键词
丹参注射液/HBOC-CHP01/复苏/失血性休克/肾脏保护Key words
Salvia miltiorrhiza injection/HBOC-CHP01/resuscitation/haemorrhagic shock/renal protection分类
医药卫生引用本文复制引用
郝莎莎,谢欣彤,李燊,张鸿辉,王红,杨成民,周文涛,刘嘉馨..丹参注射液对HBOC-CHP01复苏失血性休克大鼠肾脏保护作用的研究[J].中国输血杂志,2025,38(2):172-180,9.基金项目
中国医学科学院医学与健康科技创新工程(2021-I2M-1-060) （2021-I2M-1-060）
四川省中医药管理局面上项目(2023MS554) （2023MS554）
