中国药科大学学报2025，Vol.56Issue(1)：80-90,11.DOI:10.11665/j.issn.1000-5048.2024032802
大麦芽碱对卵清蛋白诱导的大鼠变应性鼻炎的治疗作用及机制
Therapeutic effect and mechanism of hordenine on ovalbumin-induced allergic rhinitis in rats
摘要
Abstract
To investigate the therapeutic effect and related mechanisms of hordenine on ovalbumin(OVA)-induced allergic rhinitis(AR)in rats,HE and AB-PAS staining were used to detect the improvement of pathological damage to the nasal mucosa induced by hordenine.ELISA was employed to detect the effect of hordenine on OVA-sIgE in serum and IL-4 in the nasal mucosa supernatant of rats.IHC and Western blot experiments were undertaken to examine the effect of hordenine on Th1/Th2 cell balance.Bioinformatics analysis was performed to predict pathways,which were verified by in vivo and in vitro experiments.The experimental results showed that hordenine could alleviate the behavioral manifestations of OVA-induced AR rats,alleviate nasal mucosal pathological damage caused by AR,and reduce the secretion of OVA-sIgE and IL-4.In addition,hordenine could regulate the Th1/Th2 balance.Bioinformatics analysis results showed that the potential pathway of action of hordenine on AR was the phosphoinositide 3 kinase(PI3K)/protein kinase B(Akt)signaling pathway.The in vivo experimental results showed that the expression of PI3K and p-Akt proteins in the nasal mucosa of the model group rats was significantly increased(P<0.01),and that the protein expression level was significantly decreased after the administration of hordenine,which was also confirmed by an in vitro experiment.This study suggests that hordenine may regulate Th1/Th2 cell balance through the PI3K/Akt signaling pathway,thereby exerting an alleviating effect on OVA-induced AR.关键词
变应性鼻炎/大麦芽碱/PI3K/Akt信号通路/Th1/Th2细胞平衡Key words
allergic rhinitis/hordenine/PI3K/Akt pathway/Th1/Th2 cell balance分类
药学引用本文复制引用
李俊彦,刘涛,孙芳,黄家辉,毛书振,姚静..大麦芽碱对卵清蛋白诱导的大鼠变应性鼻炎的治疗作用及机制[J].中国药科大学学报,2025,56(1):80-90,11.基金项目
山东省中医药科技项目(M-2022247) （M-2022247）
济宁医学院贺林院士新医学临床转化站科研基金项目(JYHL2021FMS13) （JYHL2021FMS13）
济宁医学院实践教学教育研究计划项目(JYSJ2022B44) This study was supported by the Traditional Chinese Medicine Science and Technology Project of Shandong Province(M-2022247) （JYSJ2022B44）
the Research Fund of Academician Lin He's Workstation of New Medicine and Clinical Translation in Jining Medical University(JYHL2021FMS13) （JYHL2021FMS13）
and the Practical Teaching and Educational Research Plan Project of Jining Medical University(JYSJ2022B44) （JYSJ2022B44）
