中国油料作物学报2025，Vol.47Issue(1)：192-203,12.DOI:10.19802/j.issn.1007-9084.2023236
大豆根瘤内生菌合成菌群对根系微生物群落的影响
Synthetic flora of soybean endophytes alter root microbial community structure and function
摘要
Abstract
To investigate the impact of synthetic bacterial flora of nodule endophytes on soybean growth and root microorganisms,this experiment used nodule endophytes isolated from soybean root nodules to construct four synthetic bacterial flora by complementation of strain functions,increase in diversity and superposition of growth-promoting effects.In the study,the bacterial groups Q1 and Q4 exhibited the best growth-promoting effects.Q1 sig-nificantly enhanced the stem length,stem fresh weight,root fresh weight and nodule number of soybean plants,in-creasing by 19.2%,44.0%,51.7%and 140.0%respectively.Q4 significantly increased the total root length,root surface area,root fresh weight and number of nodules of soybean plants,increasing by 24.3%,34.0%,40.0%and 200.0%respectively.The 16S rRNA sequencing results in the roots revealed that Bacillus and Klebsiella among the root nodule endophytes invaded soybean roots,increased the positive correlation among bacterial communities and the abundance of genes encoding genes related to ecological functions.Metagenomic sequencing results of rhizo-sphere soil showed that after inoculation with synthetic bacterial flora,beneficial bacterial genera such as Nocardioi-des and Pseudonocardia were enriched in the rhizosphere,and genes involved in nitrogen metabolism and indole-3-acetic acid(IAA)biosynthetic pathways were significantly increased,and rhizosphere bacteria to carry out biologi-cal processes such as coenzymes,amino acid transport and metabolism,carbohydrate metabolism and signal trans-duction were promoted.The results provided theoretical basis and technical support for the construction of soybean endophytic microbiome and the development of efficient microbial fertilizer.关键词
大豆/根瘤内生菌/合成菌群/促生机制/微生物肥料Key words
soybean/nodule endophytes/synthetic community/growth promotion mechanism/microbial fertilizer分类
农业科学引用本文复制引用
汪芳芳,孙秀娟,徐伟慧,陈文晶,胡云龙,王志刚..大豆根瘤内生菌合成菌群对根系微生物群落的影响[J].中国油料作物学报,2025,47(1):192-203,12.基金项目
黑龙江省杰出青年基金(JQ2023D001) （JQ2023D001）
黑龙江省重点研发计划(GA21B007) （GA21B007）
黑龙江省高校基本科研业务费项目(145209805 （）
145209810) （）
