中国药业2025，Vol.34Issue(4)：54-59,6.DOI:10.3969/j.issn.1006-4931.2025.04.013
四黄消肿散联合消肿活血散外敷对流行性感冒病毒肺炎模型小鼠的影响
Effect of External Application of Sihuang Xiaozhong Powder Combined with Xiaozhong Huoxue Powder on Model Mice with Influenza Virus-Induced Pneumonia
摘要
Abstract
Objective To investigate the effect of external application of Sihuang Xiaozhong Powder combined with Xiaozhong Huoxue Powder on the mice with viral-induced pneumonia.Methods A total of 60 mice were randomly divided into the normal control group(gavage with equal volume of physiological saline),the model group(gavage with equal volume of physiological saline),the oseltamivir group(gavage with 75 mg/kg),the Sihuang Xiaozhong Powder group(external application),the Xiaozhong Huoxue Powder group(external application),and the combination therapy group(external application+gavage with 75 mg/kg),with 10 mice in each group.The mice were anesthetized with ether,and were intranasally injected with 50 µL H1N1 virus containing one unit of median lethal dose(LD50)to replicate the mouse model of influenza virus(IFV)-induced pneumonia.The mice in the normal control group were intranasally injected with an equal volume of physiological saline.After 2 h of successful modeling,mice were given oseltamivir or physiological saline by gavage(0.2 mL/10 g);at the same time,1.5 g of the corresponding drugs were evenly applied to the back of the mice after removing hair and fixed with adhesive tape once a day for one week.The lung tissue mass of mice was weighed and the lung coefficient was calculated;hematoxylin-eosin(HE)staining was used to observe the pathological morphology of lung tissue;enzyme-linked immunosorbent assay(ELISA)was used to detect the expression levels of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6);real-time fluorescence quantitative polymerase chain reaction(RT-PCR)and Western blot were used to detect the peroxisome proliferator-activated receptor γ(PPARγ)/nuclear factor-κB(NF-κB)signaling pathway-related and apoptosis-related genes and proteins.Results Compared with those in the model group,the lung pathological changes of mice in the oseltamivir group and the combination therapy group alleviated;the lung index significantly decreased(P<0.05);the expression levels of TNF-α and IL-6 significantly reduced(P<0.05);the NF-κB,B-cell lymphoma-2-associated X protein(Bax),and Caspase-3 mRNA and protein levels significantly decreased;the PPARγand B-cell lymphoma-2(Bcl-2)mRNA and protein levels significantly increased(P<0.05).Conclusion The external application of Sihuang Xiaozhong Powder combined with Xiaozhong Huoxue Powder can further improve the pneumonia symptoms in model mice,and its mechanism may be related to the inhibition of the PPARγ/NF-κB pathway.关键词
四黄消肿散/消肿活血散/外敷/流行性感冒病毒性肺炎/过氧化物酶体增殖物激活受体γ/核因子κB/细胞凋亡Key words
Sihuang Xiaozhong Powder/Xiaozhong Huoxue Powder/external application/influenza virus-induced pneumonia/peroxisome proliferator-activated receptor γ/nuclear factor-κB/cell apoptosis分类
医药卫生引用本文复制引用
张玲,刘林洁,王雪梅,李玉梅..四黄消肿散联合消肿活血散外敷对流行性感冒病毒肺炎模型小鼠的影响[J].中国药业,2025,34(4):54-59,6.基金项目
四川省中医药管理局科学技术研究专项课题[2023MS303]. （）
