中国药业2025，Vol.34Issue(4)：59-61,3.DOI:10.3969/j.issn.1006-4931.2025.04.014
柴胡加龙骨牡蛎汤对围绝经期模型小鼠下丘脑-垂体-卵巢轴激素水平的影响
Effect of Chaihu and Longgu Muli Decoction on the Hormone Levels of Hypothalamus-Pituitary-Ovary Axis in Perimenopausal Mice
摘要
Abstract
Objective To investigate the effect of Chaihu and Longgu Muli Decoction on the hormone levels of hypothalamus-pituitary-ovary axis in perimenopausal mice.Methods A total of 40 ICR mice were randomly divided into the sham surgery group[equal volume of 1%carboxymethyl cellulose sodium(CMC-Na)solution],the model group(equal volume of 1%CMC-Na solution),the estrogen group(0.13 mg/kg),and the drug group(14 mg/g),with 10 mice in each group.After anesthesia with 0.5%pentobarbital sodium,the fallopian tubes were ligated and both ovaries were removed to replicate the perimenopausal mouse model.The mice in the sham surgery group were only removed equal amounts of adipose tissue near both ovaries.On the 8th day after modeling,electrical stimulation was performed(the mice in the sham surgery group were placed in a stimulation box for 10 min)once a day for 7 d;after daily electrical stimulation,mice in each group were given corresponding drugs or CMC-Na solution(0.2 mL/10 g)by gavage.Enzyme-linked immunosorbent assay(ELISA)was used to detect the serum estradiol(E2),follicle-stimulating hormone(FSH),luteinizing hormone(LH),and hypothalamic gonadotropin-releasing hormone(GnRH)levels in mice during day and night.Results Compared with those in the model group,the daytime hypothalamic GnRH level in the drug group significantly decreased,the daytime serum E2 level in the drug group and estrogen group significantly increased(P<0.05).Conclusion Chaihu and Longgu Muli Decoction may play a role in improving sleep by stabilizing the hypothalamus-pituitary-ovary axis and regulating hormonal secretion according to circadian rhythm.关键词
柴胡加龙骨牡蛎汤/围绝经期/小鼠/下丘脑-垂体-卵巢轴/激素水平Key words
Chaihu and Longgu Muli Decoction/perimenopause/mouse/hypothalamus-pituitary-ovary axis/hormone level分类
医药卫生引用本文复制引用
张可兴,张博,朱珊珊,吴琼,黄莉莉,刘延东..柴胡加龙骨牡蛎汤对围绝经期模型小鼠下丘脑-垂体-卵巢轴激素水平的影响[J].中国药业,2025,34(4):59-61,3.基金项目
黑龙江省中医药科研项目[ZHY2020-070]. （）
