组织工程与重建外科杂志2025，Vol.21Issue(1)：14-19,6.DOI:10.3969/j.issn.1673-0364.2025.01.003
TLR4/NF-κB信号通路在瘢痕癌组织中的表达及TAK-242对瘢痕癌细胞影响的研究
Expression of TLR4/NF-κB signaling pathway in scar cancer tissues and effect of TAK-242 on scar cancer cells
摘要
Abstract
Objective To analyze the expression of Toll like receptor 4(TLR4)and nuclear factor kappa B(NF-κB)in scar cancer tissues,and to investigate the effect of TLR4 inhibitor TAK-242 on scar cancer cells.Methods 20 cases of normal skin tissue,20 cases of hypertrophic scar tissue,and 20 cases of scar cancer tissue were collected.Immunohistochemistry and reverse transcription polymerase chain reaction(RT-PCR)were used to detect the expression of TLR4 and NF-κB protein and mRNA.The relationship between the expression of TLR4,NF-κB and clinical pathological factors such as patient gender,age,and tumor differentiation degree was analyzed.Scar cancer cells were cultured in vitro and treated with TLR4 inhibitor TAK-242.Cell proliferation activity was detected by MTT assay,and the expression of TLR4,NF-κB,MMP9,and TGF-β1 proteins was detected by Western blot assay.Results Compared with the control group,the expression of TLR4,NF-κB protein and mRNA in scar tissue and scar cancer tissue was significantly increased(P<0.05);Compared with scar tissue,the expression of TLR4,NF-κB protein and mRNA in scar cancer tissue was significantly increased(P<0.05).The expression of TLR4 and NF-κB in scar cancer tissue was related to the degree of tumor differentiation(P<0.05).After intervention with TLR4 inhibitor TAK-242,the proliferation activity of scar cancer cells was significantly reduced(P<0.05),and the protein expression of TLR4,NF-κB,MMP9,and TGF-β1 was downregulated(P<0.05).Conclusion The TLR4/NF-κB signaling pathway is involved in the pathological process of scar tissue carcinogenesis,and the TLR4 inhibitor TAK-242 can inhibit scar cancer proliferation,which is expected to become a new target for scar tissue treatment.关键词
Toll样受体4/核转录因子κB/增生性瘢痕/瘢痕癌/成纤维细胞Key words
Toll like receptor 4/Nuclear transcription factor kappa B/Hypertrophic scars/Scar cancer/Fibroblasts分类
临床医学引用本文复制引用
霍文亮,孟艳斌,田小瑞,张锦若,梁钢,王建明,白培懿,刘翠花,董玉莹,徐宽宽..TLR4/NF-κB信号通路在瘢痕癌组织中的表达及TAK-242对瘢痕癌细胞影响的研究[J].组织工程与重建外科杂志,2025,21(1):14-19,6.基金项目
山西省卫生健康委"四个一批"科研基金项目(2023XM051),山西省卫生健康委科研课题基金项目(2024183). （2023XM051）
