中国蔬菜Issue(2)：44-55,12.DOI:10.19928/j.cnki.1000-6346.2025.5001
黄瓜WIP转录因子家族基因的鉴定及表达分析
Identification and Analysis of Cucumber WIP Transcription Factor Family Genes
摘要
Abstract
The WIP transcription factor family belongs to the A1d subgroup of C2H2 zinc finger protein.At present,CsWIP1 is involved in sex determination in cucumber,but the function of WIP gene in cucumber growth and development is still unknown.This study identified 40 members of the WIP gene family in cucumber,melon,watermelon,wax gourd,bottle gourd,and zucchini.Using online websites to analyze the physicochemical properties of the WIP proteins in the Cucurbitaceae family,and conducting chromosomal localization,gene structure,and other analyses through bioinformatics.The analysis of the cis acting elements of cucumber WIP genes indicates that the WIP family genes have cis acting elements such as spatial expression,plant hormones,and photoresponse elements.Real time fluorescence quantitative PCR analysis of different tissues in cucumber revealed that CsaV3_1G014730 and CsaV3_4G031370 may play important roles in the shoot apex of cucumber;CsaV3_4G024150(CsWIP1),CsaV3_3G039140,CsaV3_7G031140,and CsaV3_5G023030 may be involved in the growth and development of female and male flowers,transmitting tract,and male gametes,suggesting that WIP gene may play an important role in the development of transport channel and regulation of leaf vein pattern in cucumber.关键词
黄瓜/WIP基因家族/转录因子/生物信息学分析/组织表达模式Key words
cucumber/WIP gene family/transcription factor/bioinformatics analysis/tissue expression pattern引用本文复制引用
韩瑞月,刘晓林,辛同旭,李森,杨学勇,孙进京..黄瓜WIP转录因子家族基因的鉴定及表达分析[J].中国蔬菜,2025,(2):44-55,12.基金项目
中国农业科学院科技创新工程项目(CAAS-ASTIP) （CAAS-ASTIP）
