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基于BSA-seq技术定位番茄花序分枝基因

孔济升 刘志强 马静 李仁 崔霞 孙帅

中国蔬菜Issue(2):56-63,8.
中国蔬菜Issue(2):56-63,8.DOI:10.19928/j.cnki.1000-6346.2024.2044

基于BSA-seq技术定位番茄花序分枝基因

Mapping of Inflorescence Branching Genes in Tomato Based on BSA-seq

孔济升 1刘志强 2马静 3李仁 2崔霞 2孙帅2

作者信息

  • 1. 青岛农业大学园艺学院,山东 青岛 266109||中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京 100081
  • 2. 中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京 100081
  • 3. 青岛农业大学园艺学院,山东 青岛 266109
  • 折叠

摘要

Abstract

Inflorescence-branching structure determines the number and size of tomato fruit,which is an important component of tomato yield and a main objective in tomato breeding.To identify and clone the key regulatory genes involved in inflorescence branching,'ST198'with complex inflorescence branching and'ST211'with simple inflorescence branching were selected as parental lines to generate an F2 mapping population.Five major quantitative trait locis(QTLs)regulating inflorescence branching were identified using BSA-seq.Analysis and validation of the major QTL,qIB12.2,revealed that J2 was the key gene regulating inflorescence branching.J2 was found to exhibit an epistatic effect on another major QTL,qIB12.4.What's more,qIB12.4 was finely mapped to the 446.7 kb region,and closely linked molecular markers were developed.Our findings provide insights into the genetic regulatory network underlying tomato inflorescence development and have important application value for precise molecular breeding of tomato.

关键词

番茄/花序形态/QTL/产量性状

Key words

tomato/inflorescence architecture/QTL/yield traits

引用本文复制引用

孔济升,刘志强,马静,李仁,崔霞,孙帅..基于BSA-seq技术定位番茄花序分枝基因[J].中国蔬菜,2025,(2):56-63,8.

基金项目

国家自然科学基金重点国际合作研究项目(32120103010),北京市种质创制和品种选育联合攻关项目(G20220628003-03),国家自然科学基金青年科学基金项目(32102403) (32120103010)

中国蔬菜

OA北大核心

1000-6346

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