中国蔬菜Issue(2):56-63,8.DOI:10.19928/j.cnki.1000-6346.2024.2044
基于BSA-seq技术定位番茄花序分枝基因
Mapping of Inflorescence Branching Genes in Tomato Based on BSA-seq
摘要
Abstract
Inflorescence-branching structure determines the number and size of tomato fruit,which is an important component of tomato yield and a main objective in tomato breeding.To identify and clone the key regulatory genes involved in inflorescence branching,'ST198'with complex inflorescence branching and'ST211'with simple inflorescence branching were selected as parental lines to generate an F2 mapping population.Five major quantitative trait locis(QTLs)regulating inflorescence branching were identified using BSA-seq.Analysis and validation of the major QTL,qIB12.2,revealed that J2 was the key gene regulating inflorescence branching.J2 was found to exhibit an epistatic effect on another major QTL,qIB12.4.What's more,qIB12.4 was finely mapped to the 446.7 kb region,and closely linked molecular markers were developed.Our findings provide insights into the genetic regulatory network underlying tomato inflorescence development and have important application value for precise molecular breeding of tomato.关键词
番茄/花序形态/QTL/产量性状Key words
tomato/inflorescence architecture/QTL/yield traits引用本文复制引用
孔济升,刘志强,马静,李仁,崔霞,孙帅..基于BSA-seq技术定位番茄花序分枝基因[J].中国蔬菜,2025,(2):56-63,8.基金项目
国家自然科学基金重点国际合作研究项目(32120103010),北京市种质创制和品种选育联合攻关项目(G20220628003-03),国家自然科学基金青年科学基金项目(32102403) (32120103010)