肿瘤防治研究2025，Vol.52Issue(2)：133-141,9.DOI:10.3971/j.issn.1000-8578.2025.24.0675
生物信息学和孟德尔随机化分析口腔鳞状细胞癌差异表达基因与患者预后的相关性
Bioinformatic and Mendelian Randomization Analyses of Correlation Between Differ-entially Expressed Genes and Prognosis of Oral Squamous Cell Carcinoma Patients
摘要
Abstract
Objective Differentially expressed genes in oral squamous cell carcinoma(OSCC)were subjected to bioinformatic and Mendelian randomization analyses to elucidate their prognostic significance in OSCC.Methods The TCGA database and dataset GSE138206 were used to screen the common differential genes of OSCC,and their relationship was analyzed by using Mendelian randomization.The prognostic value of differential genes was further analyzed by Cox risk regression.The biological function of genes with high prognostic value was further evaluated by single gene differential analysis.Results A total of 147 common differential genes were screened from the two databases.Results of two-sample Mendelian randomization showed that GREM2 was associated with the increased risk of OSCC.In addition,SH3BGRL2 was associated with a decreased risk of OSCC,and DKK1,CCL11,and HOXC6 were considered as independent prognostic markers of OSCC.The predicted results of DKK1 were consistent with the actual results.KEGG enrichment analysis indicated the potential involvement of DKK1 in arachidonic acid and linoleic acid metabolism.Furthermore,DKK1 showed positive correlations with Tgd and Th2 cells,while displaying negative associations with PDC,Cytotoxic cells,Mast cells,CD8 T cells,TFH cells,B cells,T cells,and Th17 cells.Conclusion GREM2 is associated with an increased risk of OSCC.DKK1 is highly expressed in OSCC and associated with poor prognosis,which may be involved in regulating the metabolism of arachidonic acid and linoleic acid and immune cell invasion in OSCC.关键词
生物信息/孟德尔随机化/口腔鳞状细胞癌/Cox风险回归分析Key words
Biological information/Mendelian randomization analysis/Oral squamous cell carcinoma/Cox risk regression analysis分类
临床医学引用本文复制引用
刘茂林,宋晓娜,刘雅琪,史舒瑄,宋国华..生物信息学和孟德尔随机化分析口腔鳞状细胞癌差异表达基因与患者预后的相关性[J].肿瘤防治研究,2025,52(2):133-141,9.基金项目
National Natural Science Foundation of China(No.31772551,31970513) （No.31772551,31970513）
Shanxi Province Sci-ence and Technology Innovation Talent Team Project(No.202204051002032) （No.202204051002032）
Shanxi Province Higher Edu-cation"Billion Project"Science and Technology Guidance Project(No.BYJL016) 国家自然科学基金(31772551,31970513) （No.BYJL016）
山西省科技创新人才团队项目(202204051002032) （202204051002032）
山西省高等教育"工程"科技引导(BYJL016) （BYJL016）
