中国肿瘤生物治疗杂志2025，Vol.32Issue(1)：64-72,9.DOI:10.3872/j.issn.1007-385x.2025.01.009
METTL3介导的m6A修饰调控miR-1224-5p在前列腺癌细胞增殖和迁移中的作用
Role of METTL3-mediated m6 A modification in regulating miR-1224-5p in the proliferation and migration of prostate cancer cells
摘要
Abstract
Objective:To investigate the biological role of miR-1224-5p in the proliferation,migration and apoptosis of prostate cancer cells,as well as its regulatory mechanism of expression.Methods:Human prostate cancer cells(PC3,DU145,LNCaP,22Rv1)and normal prostate epithelial RWPE-1 cells were selected for this study.The expression of miR-1224-5p in prostate cancer cells was detected by qPCR.miR-1224-5p mimic,inhibitor and corresponding negative control(NC)plasmids were transfected into PC3 and DU145 cells by liposome transfection technology,respectively.The transfection efficiency was verified by qPCR.The effects of miR-1224-5p mimic and inhibitor on cell proliferation,migration and apoptosis were detected by CCK-8 assay,plate clone formation assay,scratch healing assay and flow cytometry.The potential N6-methyladenosine(m6A)modification sites in the pri-miR-1224-5p sequence were predicted using SRAMP website,and the prediction results were verified using methylated RNA immunoprecipitation(MeRIP).The expression of methyltransferase 3(METTL3),a key methyltransferase involved in m6A modification,in prostate cancer cells was detected by qPCR.CCK-8 assay and Transwell assay were used to detect the effect of METTL3 siRNA transfection on the proliferation and migration of PC3 and DU145 cells.The regulatory effect of METTL3-mediated m6A modification on the expression of miR-1224-5p was detected by qPCR and MeRIP.Results:miR-1224-5p was upregulated in prostate cancer cells(all P<0.01).Transfection with miR-1224-5p mimic promoted the proliferation,migration and inhibited the apoptosis of PC3 and DU145 cells(P<0.05 or P<0.01).Conversely,miR-1224-5p inhibitor suppressed the proliferation,migration and induced apoptosis of PC3 and DU145 cells(P<0.05 or P<0.01).pri-miR-1224-5p contained m6A modification sites.METTL3 was highly expressed in prostate cancer cells(all P<0.01).Transfection of METTL3 siRNA inhibited the proliferation and migration of PC3 and DU145 cells(all P<0.01).METTL3-mediated m6A modification regulated the expression of miR-1224-5p.Conclusion:miR-1224-5p is upregulated in prostate cancer cells through METTL3-mediated m6A modification.Down-regulation of miR-1224-5p can inhibit the proliferation,migration and induce apoptosis of prostate cancer cells.关键词
前列腺癌/miR-1224-5p/N6-甲基腺苷/甲基转移酶3/增殖/迁移/凋亡Key words
prostate cancer/miR-1224-5p/N6-methyladenosine(m6A)/methyltransferase 3(METTL3)/proliferation/migration/apoptosis分类
临床医学引用本文复制引用
胡东来,赵梓岐,楚元奎..METTL3介导的m6A修饰调控miR-1224-5p在前列腺癌细胞增殖和迁移中的作用[J].中国肿瘤生物治疗杂志,2025,32(1):64-72,9.基金项目
国家自然科学基金(No.82160465) （No.82160465）
