中国兽药杂志2025，Vol.59Issue(2)：1-7,7.DOI:10.11751/ISSN.1002-1280.2025.2.01
牛昏睡嗜血杆菌的分离鉴定及其应用研究
Isolation and Characterization of Haemophilus somnus from Cattle and Its Application Study
摘要
Abstract
In order to clarify the pathogen characteristics of the Haemophilus somnus disease in China,the clinical samples with positive diagnosis of Haemophilus somnus were inoculated on the solid medium of Brain Heart Extraction Yeast Extraction Ointment Blood Agar.The target strains were purified and cultured on the most suitable solid-liquid medium for many generations.After 8～12 hours of liquid medium culture,the number of bacteria could reach 3×109 CFU/mL,and the minimum lethal dose for all mice was 4×108 CFU,which is highly pathogenic.After immunizing mice with inactivated vaccine prepared from the culture liquid,it can produce more than 80%immune protection effect against the epidemic pathogenic strain of Haemophilus somnus,and the safety is good.The successful isolation of this strain will play a positive effect on the development of a vaccine against bovine Hemophilus sommus disease.关键词
昏睡嗜血杆菌/分离/培养/PCR鉴定/致病力Key words
Haemophilus somnus/isolation/culture/PCR/pathogenicity分类
农业科技引用本文复制引用
刘玉梅,陈学飞,张鸣华,范文霞,赵丽霞,关平原,张彦婷,赵苏苏,赵利平,刘东霞,辛颖,方建国,杨晓婷,莫绍江..牛昏睡嗜血杆菌的分离鉴定及其应用研究[J].中国兽药杂志,2025,59(2):1-7,7.基金项目
内蒙古自治区科技重大专项牛羊支原体肺炎和病毒性呼吸道疾病的防控技术研发与示范(2021ZD0013) （2021ZD0013）
