中国兽药杂志2025，Vol.59Issue(2)：32-42,11.DOI:10.11751/ISSN.1002-1280.2025.2.05
二氢杨梅素对LPS诱导小鼠炎症损伤的保护作用研究
Study on the Protective Effects of Dihydromyricetin Against LPS-Induced Inflammatory Damage in Mice
摘要
Abstract
To investigate the preventive effect and anti-inflammatory mechanism of dihydromyricetin(DMY)against inflammatory damage in mice induced by lipopolysaccharide(LPS).RAW264.7 cells and Balb/c mice were triggered by LPS to produce in vitro and in vivo models of inflammatory injury.The phagocytic capability,damage-preventive impact,and maximum safe dosage of DMY on RAW264.7 cells were assessed using the CCK-8 and neutral red tests.RT-qPCR was used to measure the mRNA expression levels of the inflammatory markers iNOS,IL-1,IL-6,TNF-α,and genesassociated with the JAK1/STAT3 pathway in both in vitro and in vivo inflammatory damage models.The balance between Th17 and Treg was ascertained through the use of flow cytometry.In vitro experiments revealed that DMY had a better protective effect on LPS-induced inflammatory injury of cells,which significantly increased the cells'capacity to phagocytose(P<0.05),decreased NO release(P<0.05),and decreased the expression levels of inflammatory factors iNOS,IL-6,IL-10,and IL-17(P<0.05).There was a decrease in JAK1 and STAT3 expression(P<0.05).The outcomes of in vivo tests shown that every DMY group dose had a positive protective effect against LPS-induced inflammatory damage in mice and restored the mice's spleen index(P<0.05).The expression levels of genes associated with the JAK1/STAT3 pathway,iNOS,IL-1,IL-6,TNF-α,and other inflammatory indicators were reduced(P<0.05)in the spleen tissue of mice,although the balance of Th17/Treg cells was restored(P<0.05).DMY might protect mice from inflammatory damage by suppressing the JAK1/STAT3 signaling pathway and Th17/Treg balance,encouraging macrophage proliferation,preventing the production of inflammatory markers,and restoring the spleen organ index.关键词
二氢杨梅素/脂多糖/炎症损伤/JAK1/STAT3信号通路/Th17/Treg平衡Key words
Dihydromyricetin/LPS/Inflammatory damage/JAK1/STAT3 signaling pathway/Th17/Treg balance分类
农业科技引用本文复制引用
王宁,闫普普,柳丹,吴利军,张付贤,汤峰,郭利伟,何斌..二氢杨梅素对LPS诱导小鼠炎症损伤的保护作用研究[J].中国兽药杂志,2025,59(2):32-42,11.基金项目
湖北省支持种业高质量发展资金项目(HBZY2023B006-04) （HBZY2023B006-04）
