中国兽药杂志2025，Vol.59Issue(2)：50-55,6.DOI:10.11751/ISSN.1002-1280.2025.2.07
猪瘟病毒片状载体培养生产工艺研究
Research on the Production Process of Classical Swine Fever Virus Flake Carrier Culture
许冬 1商琦 1侯丹丹 1张辰羽 1张星 1虞慎义 1张肖玲 1曹锋1
作者信息
- 1. 安徽东方帝维生物制品股份有限公司,安徽亳州 236800
- 折叠
摘要
Abstract
To enhance the viral content of classical swine fever antigen,mitigate the side effects associated with classical swine fever vaccine,and reduce production costs,we investigated the production process of classical swine fever virus flake carrier culture.ST cells were inoculated onto flake carriers for cultivation,and a series of experimental methods were employed to optimize parameters,including cell inoculation density,toxic dose,initial and subsequent harvest time,continuous harvest interval time,number of consecutive harvests,and immune efficacy of the final product.Subsequently,the optimal production process was determined.The results revealed that the culture was carried out with a density of 0.5×106/mL on flake carriers.After 72 hours,the original culture medium was replaced with a cell maintenance medium containing 7%classical swine fever virus to sustain the culture.Medium replacement and harvest were performed every 72 hours,resulting in a virus content of classical swine fever antigen reaching 1×107 RID/mL,a significant increase compared to the traditional bottle transfer production process by 10 to 20 times.The prepared product exhibited excellent immune effects.This study successfully established the optimized production process for the cultivation of classical swine fever virus in flake carriers,verified the immune effect of antigen cultivation on such carriers,and provided new ideas for the production and research of high-quality classical swine fever vaccines.关键词
猪瘟病毒/片状载体/病毒含量/免疫效果Key words
classical swine fever virus/flake carrier/virus content/immune efficacy分类
农业科技引用本文复制引用
许冬,商琦,侯丹丹,张辰羽,张星,虞慎义,张肖玲,曹锋..猪瘟病毒片状载体培养生产工艺研究[J].中国兽药杂志,2025,59(2):50-55,6.
