浙江大学学报（农业与生命科学版）2025，Vol.51Issue(1)：67-79,13.DOI:10.3785/j.issn.1008-9209.2024.11.091
百合原生质体分离培养和瞬时转化
Isolation,cultivation,and transient transformation of lily protoplasts
摘要
Abstract
Protoplasts are important receptors for genetic transformation and gene function verification.Lily is an important ornamental,edible,and medicinal plant worldwide.The isolation and cultivation system for lily protoplasts is still incomplete.In this study,taking Lilium pumilum and Lilium formosanum×Lilium longiflorum var.scabrum as materials,the isolation,cultivation,and transient transformation of lily protoplasts were studied.The results revealed that lily leaves and embryogenic callus tissues were both excellent materials for isolating protoplasts.The optimal solution for isolating protoplasts from sterile plantlet leaves was cell protoplast wash medium(CPW)+1.0%-2.0%cellulase RS+0.5%macerozyme R-10+0.10%pectinase Y-23+12-14 g/L D-mannitol.The optimal solution for isolating protoplasts from embryogenic callus tissues was CPW+2.0%cellulase RS+0.60%pectinase Y-23+12-14 g/L D-mannitol.Protoplasts isolated from leaves were excellent receptors for transient transformation,with a transformation efficiency of 34.0%-36.7%.Protoplasts isolated from embryogenic callus tissues of Lilium formosanum×Lilium longiflorum var.scabrum had strong division ability.In a solid-liquid double-layer culture medium with MS(Murashige-Skoog,containing 206.25 mg/L NH4NO3)+60 g/L glucose and a cultivation density of 2×105 cells/mL,callus tissues were formed after 70 days of cultivation.关键词
细叶百合/新铁炮百合/原生质体培养/瞬时转化Key words
Lilium pumilum/Lilium formosanum×Lilium longiflorum var.scabrum/protoplast culture/transient transformation分类
农业科技引用本文复制引用
何珊珊,李宏宇,马月,孙红梅..百合原生质体分离培养和瞬时转化[J].浙江大学学报（农业与生命科学版）,2025,51(1):67-79,13.基金项目
辽宁省种质创新藏粮于技专项计划项目(2023JH1/10200010) （2023JH1/10200010）
国家现代农业产业技术体系建设专项(CARS-23). （CARS-23）
