浙江大学学报（农业与生命科学版）2025，Vol.51Issue(1)：118-127,10.DOI:10.3785/j.issn.1008-9209.2024.12.022
郁金香二氢黄酮醇4-还原酶基因TgDFR1和TgDFR2的克隆与功能鉴定
Cloning and functional identification of the dihydroflavonol 4-reductase genes TgDFR1 and TgDFR2 in tulips
摘要
Abstract
The dihydroflavonol 4-reductase(DFR)gene plays a crucial role in the formation of flower color.In this study,two DFR genes were isolated from Tulipa gesneriana'Strong Fire'.Sequence analysis showed that TgDFR1 and TgDFR2 encode putative proteins of 365 and 422 amino acids,respectively.The expression levels of these genes at different developmental stages of the flower were examined,revealing that TgDFR1 and TgDFR2 were significantly upregulated at the second stage of flower development(coloring).While the expression level of TgDFR1 declined at the third(early flowering)and fourth(full flowering)stages,TgDFR2 maintained high expression levels,showing a positive correlation with the accumulation of anthocyanins.Overexpression of TgDFR1 and TgDFR2 in tobacco showed that the expression levels of TgDFR1 and TgDFR2 and anthocyanin content were significantly increased in transgenic lines.Further analysis of the expression of the flower color related genes indicated significant upregulation of NtDFR and significant downregulation of genes encoding chalcone synthase(CHS),chalcone isomerase(CHI),flavonol synthase(FLS)and flavanone 3-hydroxylase(F3H)in transgenic tobacco plants compared with the empty vector control plants.In summary,TgDFR1 and TgDFR2 play key roles in the accumulation of anthocyanins in tulips.关键词
郁金香/二氢黄酮醇4-还原酶基因/花青素苷/基因功能Key words
Tulipa gesneriana/dihydroflavonol 4-reductase(DFR)gene/anthocyanins/gene function分类
农业科技引用本文复制引用
张恒滨,黄玲,胡先梅,梁泽慧,王艳平,产祝龙,向林..郁金香二氢黄酮醇4-还原酶基因TgDFR1和TgDFR2的克隆与功能鉴定[J].浙江大学学报（农业与生命科学版）,2025,51(1):118-127,10.基金项目
湖北省重点研发计划项目(2023BBB139) （2023BBB139）
江西省"双千计划"项目(S2021DQKJ2885) （S2021DQKJ2885）
云南省高校服务重点产业科技项目(FWCY-QYCT2024001). （FWCY-QYCT2024001）
