安徽农业科学2025，Vol.53Issue(5)：93-95,105,4.DOI:10.3969/j.issn.0517-6611.2025.05.018
基于trnL-trnF基因序列鉴别魔芋属核心种质资源
Identification of Core Germplasm Resources of Amorphophallus Based on trnL-trnF Gene Sequence
摘要
Abstract
24 Amorphophallus plants such as A.mueller,A.pmuelleraeoniifolius'Yellow',A.yunnesis,A.kachinensis,A.yuleonsis,A.sinensis,A.krausei,A.asterostigmatus,A.albus,A.konjac,hybrid konjac were used as intraspecific and interspecific plants for identifica-tion.The extracted DNA was amplified by PCR with chloroplast gene trnL-trnF,and sequenced in the first generation of the two-terminal se-quencing strategy.The sequencing results were compared and analyzed with BioEdit,and the sequences were compared on NCBI.Finally,the phylogenetic tree was constructed with MEGA 8.0.The results showed that the DNA amplification efficiency of 24 species of Amorphophallus was 100%,and the best identity in NCBI was 96.31%-100%,all belong to Amorphophallus.The results showed that the primers of trnL-trnF had good universality and high specificity,and the primers could be used to quickly identify species and analyze their genetic relationship,pro-viding reference for the study of parental selection in konjac cross breeding.关键词
魔芋/trnL-trnF/系统进化树Key words
Konjac/trnL-trnF/Phylogenetic tree分类
农业科技引用本文复制引用
王孟,杨朝柱,覃宇,赵青华..基于trnL-trnF基因序列鉴别魔芋属核心种质资源[J].安徽农业科学,2025,53(5):93-95,105,4.基金项目
湖北省农业科技创新中心项目(2016-620-000-001-062). （2016-620-000-001-062）
