中医康复2025，Vol.2Issue(4)：43-50,8.DOI:10.19787/j.issn.2097-3128.2025.04.008
推拿对神经病理性疼痛大鼠脊髓背角神经元形态及Wnt和β-catenin蛋白表达的影响
The Effect of Massage on the Expression of Spinal Wnt and β-catenin Proteins in Rats with Neuropathic Pain
摘要
Abstract
Objective:To investigate the effect of Tuina therapy on the expression of synaptic proteins Wnt and β-catenin in the spinal dorsal horn of rats with neuropathic pain.Methods:Twenty-four SD rats were randomly divided into three groups:blank group,model group,and Tuina group,with eight rats in each group.The chronic constrictive injury(CCI)model was used to induce neuro-pathic pain in rats.Starting on the 4th day after modeling,rats in the Tuina group received kneading and rubbing interventions for 14 consecutive days.No intervention was applied to the blank and model groups.Pain behavioral changes were assessed by measuring paw withdrawal threshold(PWT)and paw withdrawal latency(PWL)on the 4th,10th,14th,and 17th days post-modeling.Hematoxylin-eosin(HE)staining was used to observe morphological changes in neurons in the right spinal dorsal horn.β-catenin protein expression was detected via immunofluorescence staining,and Western blot was used to analyze the expression levels of Wnt and β-catenin proteins.Results:Behavioral results showed that the PWL and PWT of rats in the model group were significantly re-duced,indicating increased pain sensitivity.After Tuina intervention,the PWL and PWT of rats in the Tuina group were significantly improved,demonstrating that Tuina effectively alleviates neuropathic pain(P<0.001).Immunofluorescence staining showed that β-catenin positive expression in the right dorsal horn of the spinal cord was reduced in the Tuina group compared to the model group(P<0.001).HE staining revealed severe neuronal damage in the right dorsal horn of the spinal cord in the model group,with some neu-ronal cell bodies showing shrinkage,slightly irregular nuclear morphology,and loose tissue structure with increased intercellular spaces.In the Tuina group,neuronal damage was alleviated,with some neurons showing partial recovery in size,more intact and plump cell bodies,clearer background tissue structure,more organized cell arrangement,and reduced intercellular spaces.Western blot results indicated increased expression of Wnt and β-catenin proteins in the right dorsal horn of the spinal cord in CCI rats,while Tuina treatment reduced the expression levels of Wnt and β-catenin proteins(P<0.01,P<0.001).Conclusion:Tuina alleviates neuro-nal damage in the spinal dorsal horn of neuropathic pain model rats.The mechanism of pain relief may involve upregulation of Wnt and β-catenin expression.关键词
神经病理性疼痛/Wnt/β-catenin/推拿/突触可塑性Key words
neuropathic pain/Wnt/β-catenin/Tuina/synaptic plasticity分类
中医学引用本文复制引用
蔡恒昌,陈水金,林志刚,陈乐春,黄红叶,蒋晶晶,张幻真,陈进城..推拿对神经病理性疼痛大鼠脊髓背角神经元形态及Wnt和β-catenin蛋白表达的影响[J].中医康复,2025,2(4):43-50,8.基金项目
国家自然科学基金项目(82174523、82105039、82205303) （82174523、82105039、82205303）
福建省杰出青年基金项目(2023J06037、2024J01141) （2023J06037、2024J01141）
中国康复医学会科研课题重点项目(KFKT-2024-KY-005) （KFKT-2024-KY-005）
福建省卫生健康中青年领军人才研修培养项目 （）
