北京林业大学学报2025，Vol.47Issue(3)：19-27,9.DOI:10.12171/j.1000-1522.20240103
基于转录组的ABA介导的旱柳枝条失水响应
Transcriptome based ABA mediated dehydration response of Salix matsudana branches
摘要
Abstract
[Objective]ABA is a key hormone for drought stress in plants.This study aimed to explore the key functional genes and related pathways for ABA-mediated dehydrated response of Salix matsudana for breeding superior drought-resistant varieties through molecular design breeding.[Method]In this study,we investigated the differential response of xylem transcriptome of S.matsudana branches dehydrated to varied water potentials after being soaked in the sodium tungstate(ABA synthesis inhibitor)solution by transcriptome sequencing,using S.matsudana isolated branches as materials.[Result](1)There were 5 672 significantly different genes between the deionized water-soaked S.matsudana non-dehydrated branches and those dehydrated to P50 water potential branches,and 5 033 significantly different genes between the sodium tungstate solution-soaked branches and deionized water-soaked dehydrated to P50 water potential branches.(2)Plant hormone signal transduction,carotenoid biosynthesis and starch and sucrose metabolism pathways were key pathways in the dehydrated response of S.matsudana branches after being soaked in the sodium tungstate solution.(3)Soaking Salix matsudana in deionized water until P50 water potential resulted in significantly increased expression levels of PP2C family genes(P2C03,P2C51),ABA receptor regulatory component genes(PYL8),and ABA response element binding protein genes(AI5L5)in the plant hormone signaling pathway compared with non dehydrated branches.In the biosynthesis pathway of carotenoids,the expression levels of carotenoid isomerase gene(CRTSO)and abscisic aldehyde oxidase gene 3(ALDO3)were significantly upregulated.In the starch and sucrose metabolism pathways,the expression levels of glycosyl hydrolase family genes(INVA,E133),α-amylase like 3 gene(AMY3),trehalose phosphatase gene(TPS5),sucrose phosphate synthase 1 gene(SPSA1),etc.were significantly upregulated,while the expression level of starch synthase gene(SSY1)was significantly downregulated.The related genes were opposite expressed in the dehydrated branches of S.matsudana after being soaked in the sodium tungstate solution.[Conclusion]In this study,the drought stress-related pathways and the important functional genes of S.matsudana are explored,which provides the research basis for revealing drought stress response of S.matsudana.关键词
旱柳/水势/转录组/ABA/淀粉水解/干旱响应/差异表达基因Key words
Salix matsudana/water potential/transcriptome/ABA/starch hydrolysis/drought stress response/DEGs分类
林学引用本文复制引用
胡耀芳,李奥,殷佳慧,王元成,邹竣竹,巨关升,刘俊祥,孙振元..基于转录组的ABA介导的旱柳枝条失水响应[J].北京林业大学学报,2025,47(3):19-27,9.基金项目
国家自然科学基金项目(32071729). （32071729）
