吉林大学学报（医学版）2025，Vol.51Issue(1)：34-43,10.DOI:10.13481/j.1671-587X.20250105
硫化氢合成酶CBS和CSE对乳腺癌细胞恶性生物学行为的影响
Effects of hydrogen sulfide synthase CBS and CSE on malignant biological behaviour of breast cancer cells
摘要
Abstract
Objective:To investigate the expressions of cystathionine-β-synthase(CBS)and cystathionine-γ-lyase(CSE)and their effects on the malignant biological behaviours of breast cancer cells,and to elucidate their mechanisms.Methods:The breast cancer tissue and paracancerous normal tissue from 15 cases of patients were selected,and RT-qPCR and Western blotting methods were used to detect the mRNA and protein expression levels of CBS and CSE in breast cancer tissue,paracancerous normal tissue,MCF-7 cells,and MDA-MB-231 cells.The MCF-7 cells were divided into siNC group(transfected with siNC)and siCBS group(transfected with siCBS),and the MDA-MB-231 cells were divided into ovNC group(transfected with CSE over-expression empty plasmid)and ovCSE group(transfected with CSE over-expression plasmid).CCK8 assay was used to detect the proliferation activities of breast cancer cells in various groups,Transwell assay was used to detect the numbers of migration and invasion cells in various groups,and Western blotting method was used to detect the protein expression levels of E-cadherin,N-cadherin and Vimentin proteins in the breast cancer cells in various groups.Results:Compared with paracancerous normal tissue,the expression levels of CBS and CSE mRNA and proteins in breast cancer tissue were increased(P＜0.05 or P＜0.01).Compared with MDA-MB-231 cells,the CBS mRNA expression level in the MCF-7 cells was increased(P＜0.05);compared with MCF-7 cells,the expression level of CSE protein in the MDA-MB-231 cells was decreased(P＜0.05).Compared with siNC group,the proliferation activity,the numbers of migration and invasion cells,the expression levels of N-cadherin and Vimentin proteins in the MCF-7 cells in siCBS group were significantly decreased(P＜0.05),and the expression level of E-cadherin protein was increased(P＜0.05).Compared with ovNC group,the proliferation activity,the numbers of migratoin and invasion cells,and the expression levels of N-cadherin and Vimentin proteins in the MDA-MB-231 cells in ovCSE group were increased(P＜0.05),while the expression level of E-cadherin protein was significantly decreased(P＜0.05).Conclusion:The expressions of CBS and CSE are upregulated in breast cancer tissue,and high levels of CBS and CSE promote proliferation,migration,invasion and epithelial-mesenchymal transition(EMT)of breast cancer cells.关键词
胱硫醚-β-合酶/胱硫醚-γ-裂解酶/硫化氢/乳腺肿瘤/细胞增殖/细胞迁移Key words
Cystathionine-β-synthase/Cystathionine-γ-lyase/Hydrogen sulfide/Breast neoplasms/Cell proliferation/Cell migration分类
临床医学引用本文复制引用
赵蒙蒙,胡建明,王雅璐,许宇翔,杨凯歌,曹玉文,周文虎,费晶,王雯,罗成华..硫化氢合成酶CBS和CSE对乳腺癌细胞恶性生物学行为的影响[J].吉林大学学报（医学版）,2025,51(1):34-43,10.基金项目
国家自然科学基金项目(82103290) （82103290）
新疆维吾尔自治区人力资源和社会保障厅"天池英才"计划项目(CZ003103) （CZ003103）
新疆生产建设兵团科技研究指导项目(2022ZD003,2023ZD029) （2022ZD003,2023ZD029）
新疆生产建设兵团科技计划项目(2024DA041) （2024DA041）
石河子大学国家大学生创新创业训练计划项目(202410759083) （202410759083）
石河子大学青年创新拔尖人才项目(CXBJ202207) （CXBJ202207）
新疆生产建设兵团重点领域科技攻关项目(2023AB0S8) （2023AB0S8）
