长春中医药大学学报2025，Vol.41Issue(3)：272-277,6.DOI:10.13463/j.cnki.cczyy.2025.03.009
肾浊方通过抑制肾小球内皮细胞异常血管新生治疗早期糖尿病肾脏疾病的作用机制研究
Study on the mechanism of Shenzhuo Formula in treating early diabetes nephropathy by inhibiting the abnormal angiogenesis of glomerular endothelial cells
摘要
Abstract
Objective To investigate the effect of Shenzhuo Formula on glomerular endothelial cell migration and angiogenesis,and its potential mechanisms in the treatment of early diabetic kidney disease(DKD).Methods The effect of Shenzhuo Formula on DKD was studied in spontaneous type 2 diabetes db/db mice(with kidney injury at the age of 8 weeks).The study used mouse glomerular endothelial cells(MGECs)to establish an in vitro high glucose model to investigate the effect of Shenzhuo Formula on the migration and angiogenesis ability of MGECs.Results Shenzhuo Formula ameliorated kidney damage in db/db mice.Western blot and immunohistochemistry results showed that the expression levels of vascular endothelial growth factor A(VEGFA),vascular endothelial growth factor receptor-2(VEGFR2),aquaporin-1(AQP1),and platelet endothelial cell adhesion molecule(CD31)proteins were incresed in db/db mice,while treatment with Shenzhuo Formula reduced the expression of these proteins.In the high glucose model of MGECs,Shenzhuo Formula reversed the enhanced migration and angiogenesis ability of MGECs in a high glucose environment by altering the expression of VEGFA,VEGFR2,AQP1,and CD31.In addition,the therapeutic effect of Shenzhuo Formula was enhanced with the increase of concentrations of Shenzhuo formula.Conclusion The study indicates that Shenzhuo Formula can improve early renal injury in DKD by inhibiting glomerular endothelial cell migration and angiogenesis,thus further clarifying the mechanisms of action of Shenzhuo formula.Additionally,this study finds increased expression of AQP1 in DKD,providing new perspective for further investigating the potential pathogenesis of DKD.关键词
肾浊方/糖尿病肾脏疾病/异常血管新生/血管内皮生长因子 A/水通道蛋白1Key words
Shenzhuo formula/diabetic kidney disease/abnormal angiogenesis/vascular endothelial growth factor A/aquaporin 1分类
中医学引用本文复制引用
张家义,李玉杰,陈雅欣,张玉凤,王希,李伟,刘雪梅,万倩慧,孙振华,王文波..肾浊方通过抑制肾小球内皮细胞异常血管新生治疗早期糖尿病肾脏疾病的作用机制研究[J].长春中医药大学学报,2025,41(3):272-277,6.基金项目
山东省自然科学基金(ZR2023MH300) （ZR2023MH300）
中国博士后科学基金(2021M692750) （2021M692750）
泰山学者项目(tsqn202211356) （tsqn202211356）
