常熟理工学院学报2025，Vol.39Issue(2)：38-45,8.
金针菇多糖纯化、乙酰化修饰及其抗氧化活性研究
Purification,Acetylation Modification and Antioxidant Activity of Polysaccharide from Flammulina Velutipes
摘要
Abstract
Flammulina velutipes was used as materials.Polysaccharides from F.velutipes were extracted by hot water extraction.A novel polysaccharide code FVP-0.6-c was purified by DEAE-Cellulose 52 ion exchange column chromatography and Sephadex G-200 gel column chromatography.FVP-0.6-c was actylated by acetic anhydride to prepare acetylated FVP-0.6-c(Ac-FVP-0.6-c).Degree of substitution was used as an index;the effect of reaction time,reaction temperature and amount of acetic anhydride on degree of substitution were investigated.Based on the single factor experiment,the process of acetylation modification of FVP-0.6-c was optimized by response surface Box-Benhnken design.The effect of acetylation modification on the antioxidant activity of FVP-0.6-c was studied.The results showed that the optimum conditions of FVP-0.6-c acetylation are as follows:The amount of acetic anhydride was 3.3 mL.Reaction time was 3.1 h.Reaction temperature was 64℃.Under the optimum condition,the degree of substitution was 0.416.Compared with FVP-0.6-c,the scavenging effect of Ac-FVP-0.6-c on hydroxyl radical,DPPH radical and superoxide anion radical was significantly enhanced.关键词
金针菇多糖/乙酰化/修饰/抗氧化Key words
polysaccharide from Flammulina velutipes/acetylation/modification/antioxidant分类
轻工业引用本文复制引用
徐寒雯,陈义勇,柏雪,周南南,金源,荣可瑜,张宁,赵时曼,陆庆萍,李新娜..金针菇多糖纯化、乙酰化修饰及其抗氧化活性研究[J].常熟理工学院学报,2025,39(2):38-45,8.基金项目
2023年度江苏省大学生创新创业计划项目"金针菇多糖乙酰化修饰及其抗氧化活性"(202310333110Y) （202310333110Y）
