常熟理工学院学报2025，Vol.39Issue(2)：54-60,7.
鸡CTCF基因真核表达载体的构建及其对脂类代谢作用的研究
Construction of Chicken CTCF Gene Eukaryotic Expression Vector and Its Effect on Lipid Metabolism
摘要
Abstract
The CCCTC-binding factor(CTCF)is a multifunctional and highly conserved transcriptional regulator involved in processes such as transcriptional control,chromatin structure maintenance,and cellular differentiation.This study utilized RT-qPCR to analyze CTCF gene expression across various chicken tissues,revealing elevated expression levels in the brain and heart.A eukaryotic overexpression vector containing the CTCF gene was constructed and transfected into the Leghorn male hepatoma(LMH)cell line,successfully expressing the CTCF-Myc fusion protein.Oil Red O staining and colorimetric analysis demonstrated that CTCF overexpression in LMH cells significantly increased lipid droplet accumulation and total triglyceride(TG)levels(P<0.01).Concurrently,expression of Lipin 1(LIPIN1)was significantly downregulated(P<0.01),while expressions of Sterol Regulatory Element-Binding Protein 1(SREBP1),Fatty Acid Synthase(FASN),and Acetyl-CoA Carboxylase Alpha(ACACA)were significantly upregulated(P<0.05 or P<0.01).These results suggest that CTCF gene may promote lipid accumulation by regulating the expression of genes involved in lipid metabolism.关键词
CCCTC结合因子/鸡肝癌细胞/脂滴沉积/甘油三酯/脂类代谢相关基因Key words
CCCTC-binding factor/leghorn male hepatoma/lipid droplet deposition/triglycerides/lipid metabolism-related genes分类
畜牧业引用本文复制引用
夏磊,魏志恒,裴军,刘世昊,周博,徐璐,郁建锋,顾志良..鸡CTCF基因真核表达载体的构建及其对脂类代谢作用的研究[J].常熟理工学院学报,2025,39(2):54-60,7.基金项目
江苏省自然科学基金项目"RNA去甲基化酶FTO对鸡肝脏细胞脂类代谢和脂肪沉积作用的机制研究"(BK20191476) （BK20191476）
