动物营养学报2025，Vol.37Issue(2)：938-949,12.DOI:10.12418/CJAN2025.082
胚蛋注射鼠李糖乳杆菌对黄羽肉鸡早期生长性能、肠道健康、血清生化指标和盲肠微生物区系的影响
Effects of in Ovo Injection of Lactobacillus rhamnosus on Growth Performance,Intestinal Health,Serum Biochemical Indices and Cecal Microflora of Yellow-Feathered Broilers at Early Stage
摘要
Abstract
This experiment was conducted to investigate the effects of in ovo injection of Lactobacillus rhamno-sus(LR)on growth performance,intestinal health,serum biochemical indices and cecal microflora of yellow-feathered broilers at early stage.A randomized complete block design was applied in this study.A total of 240 hatching eggs of yellow-feathered broilers with similar weight[(58.70±0.25)g]were randomly allocated in the same incubator with eight layers(layer served as block).Each layer contained 3 rows of eggs.On 18.5 day of incubation,hatching eggs were randomly injected 100 μL of phosphate buffer(control group),100 μL of low dose LR(102 CFU,low-dose LR group),or 100 μL of high dose LR(104 CFU,high-dose LR group)at amnion.There were 8 replicates in each group and 10 eggs in each replicate.At hatch,the in ovo injected birds were transferred to corresponding cage according the block order and continually fed the same diet until 21 days of age.There were 8 replicates(cages)in each group and 9 to 10 birds in each replicate.The results showed as follows:1)the body weight at 21 days of age and average daily gain from 1 to 21 days of age in both LR injected groups were significantly higher than those in the control group(P＜0.05).2)The duodenal,jejunal and ileal villus heights and villus widths at 21 days of age in the LR injected groups were significantly increased compared with the groups(P＜0.05).3)As compared to the control group,the contents of serum albumin and total protein in low-dose LR group were significantly increased(P＜0.05).4)16S rRNA ampli-con sequencing data indicated that in ovo injection of LR did not significantly affect cecal microbial α diversity(Shannon index,Simpson index,Chao index and Sobs index,P＞0.05).However,the β diversity analysis indicated the cecal microbial structure changed significantly(P＜0.05).Compared with the control group,the high dose LR injection significantly increased the relative abundance of UCG-005(P＜0.05),and the low dose LR injection significantly increased the relative abundance of Lachnoclostridium(P＜0.05).In conclusion,in ovo injection of LR(102 to 104 CFU)can improve the early growth rate of yellow-feathered broilers by impro-ving the intestinal morphology and increasing the proportion of intestinal beneficial bacteria.[Chinese Journal of Animal Nutrition,2025,37(2):938-949]关键词
胚蛋注射/鼠李糖乳杆菌/黄羽肉鸡/生长性能/微生物区系Key words
in ovo injection/Lactobacillus rhamnosus/yellow-feathered broilers/growth performance/mi-croflora分类
农业科技引用本文复制引用
于潇,姚杰,苏琳洁,吴亚妮,马逸轩,黄艳玲,王茜..胚蛋注射鼠李糖乳杆菌对黄羽肉鸡早期生长性能、肠道健康、血清生化指标和盲肠微生物区系的影响[J].动物营养学报,2025,37(2):938-949,12.基金项目
国家自然科学基金项目(32102591) （32102591）
西南民族大学中央高校基本科研业务费专项资金项目(ZYN2023002) （ZYN2023002）
