动物营养学报2025，Vol.37Issue(2)：1022-1032,11.DOI:10.12418/CJAN2025.089
黄豆苷元和藤茶黄酮组合在蛋鸡卵巢颗粒细胞上的抗氧化作用及其对产蛋后期蛋鸡生产性能的影响
Antioxidant Effects of Daidzein and Dihydromyricetin Combination on Ovarian Granulosa Cells of Laying Hens and Its Effects on Performance of Laying Hens during Later Laying Period
摘要
Abstract
The objective of this study was to investigate the different ratios of antioxidant effects of daidzein(DA)and dihydromyricetin(DHM)combination in diets on ovarian granulosa cells(GCs)of laying hens and its effects on performance of laying hens during later laying period.The ovarian GCs were treated with tert-butyl hydroperoxide(TBHP)to establish an oxidative stress model,the cell experiment was divided into 5 groups:control group(group Ⅰ),TBHP group(group Ⅱ),TBHP+DA∶DHM(7∶3)group(group Ⅲ),TBHP+DA∶DHM(8∶2)group Ⅱ(group Ⅳ)and TBHP+DA∶DHM(9∶1)group Ⅲ(group Ⅴ).Animal experiment:a total of 192 Jingfen No.1 laying hens at 75 weeks of aged were divided into 2 groups according to similar egg production,with 6 replicates per group and 16 hens per replicate.The control group was fed a basal diet,while the experimental group was fed the basal diet supplemented with 300 mg/kg DA∶DHM(7∶3)combination.The pre-experimental period lasted for 1 week,and the experimental period lasted for 12 weeks.The results showed as follows:1)in the cell experiment,compared with group Ⅰ,the activities of su-peroxide dismutase(SOD),glutathione peroxidase(GSH-Px),catalase(CAT)and total antioxidant capacity(T-AOC)in GCs of group Ⅱ were significantly decreased(P＜0.05),and the malondialdehyde(MDA)con-tent was significantly increased(P＜0.05);compared with group Ⅱ,the activities of SOD,GSH-Px,CAT and T-AOC in GCs of groups Ⅲ,Ⅳ and Ⅴ were significantly increased(P＜0.05),and the MDA content was significantly decreased(P＜0.05).2)In animal experiment,compared with the control group,the egg production of experimental group was significantly increased(P＜0.05),the feed-to-egg ratio was significantly decreased(P＜0.05),the ovarian white follicle number and T-AOC were significantly increased(P＜0.05),the ovarian MDA content was significantly decreased(P＜0.01),the serum estradiol(E2)content was signifi-cantly decreased(P＜0.05),and the contents of progesterone(P4),follicle stimulating hormone(FSH)and luteinizing hormone(LH)in serum were significantly increased(P＜0.01).In conclusion,the combination addition of DA and DHM at a 7∶3 ratio can enhance the endogenous antioxidant enzyme activity in ovarian GCs of laying hens,increase the ovarian antioxidant capacity of laying hens during the later laying period,reduce MDA content,and increase serum P4 and FSH contents,thereby improving the performance of laying hens in the later laying period.[Chinese Journal of Animal Nutrition,2025,37(2):1022-1032]关键词
蛋鸡/卵巢颗粒细胞/产蛋后期/氧化应激/生产性能Key words
laying hens/ovarian granulosa cells/later laying period/oxidative stress/performance分类
农业科技引用本文复制引用
鄂晓迪,胡友军,赵晓南,文伟,程皇座,陈杰梅,王龙书,苟钟勇..黄豆苷元和藤茶黄酮组合在蛋鸡卵巢颗粒细胞上的抗氧化作用及其对产蛋后期蛋鸡生产性能的影响[J].动物营养学报,2025,37(2):1022-1032,11.基金项目
广州市科技计划项目(202206010168,2024E04J0562) （202206010168,2024E04J0562）
国家重点研发计划项目(2021YFD1300404,2022YFD1300502) （2021YFD1300404,2022YFD1300502）
广东省基础与应用基础研究基金项目(2024A1515012121) （2024A1515012121）
广东省自然科学基金项目(2022A1515012069) （2022A1515012069）
广东省农业科学院科技计划项目(202106TD) （202106TD）
广东省农业科学院科技人才引进专项资金项目(R2021YJ-YB3012) （R2021YJ-YB3012）
广东省农业科学院青年科技人员指导协议(R2021QD-024) （R2021QD-024）
广东省畜禽育种与营养研究重点实验室开放课题(2021SZ03) （2021SZ03）
