动物营养学报2025，Vol.37Issue(2)：1059-1070,12.DOI:10.12418/CJAN2025.092
酵母培养物对奶牛体外瘤胃甲烷排放、发酵参数及微生物区系的影响
Effects of Yeast Culture on in Vitro Rumen Methane Emission,Fermentation Parameters and Microbiota of Dairy Cows
摘要
Abstract
The aim of this study was to investigate the effects of yeast cultures with different functional constit-uents on rumen methane emission,fermentation parameters and microbiota of dairy cows under in vitro condi-tion.Three yeast culture were used in this study,mycosorb,actigen and XP for setting up four groups(con-trol,Myc,Act and XP groups),with six replicates in each group,the test groups were supplemented with 0.075 g of yeast culture on the basis of 100 g of substrate of control group,respectively.The AGRS-Ⅲ system was used for monitoring the fermentation for 48 h.The amount of gas production and curve parameters were re-corded,and the gas and fermentation fluid were collected to analyze the gas components microbiota and fer-mentation parameters and microbiota.Results showed as follows:1)total gas production was significantly low-er in the Myc group compared with the control group(P＜0.05);rumen methane yield was significantly lower in the Act group compared with the control group(P＜0.05).2)Ammoniacal nitrogen content was significant-ly lower in the Act group compared with the control group(P＜0.05);the addition of Myc and Act significant-ly decreased the contents of acetate,isobutyric,butyrate,isovalerate,valerate,and the total volatile fatty acids(P＜0.05);XP significantly increased the content of butyrate compared with the control group(P＜0.05).3)Compared with the control group,the microbiota Chao1 index was significantly lower in the XP group(P＜0.05).At the phylum level,the top three dominant phyla were Bacteroidota,Bacillota and Pseudomonadota;compared with the control group,XP significantly increased the relative abundance of the Bacillota(P＜0.05)and decreased the relative abundance of the Pseudomonadota(P＜0.05).At the genus level,compared with the control group,the relative abundance of the Rikenellaceae_RC9_gut_group was significantly decreased in the Act group and XP group(P＜0.05),and the relative abundance of the Christensenellaceae_R-7_group was sig-nificantly increased(P＜0.05);compared with the control group,the relative abundance of Treponema_2 and Fibrobacter increased significantly in the Act group(P＜0.05),and the relative abundance of Succinivibrio was significantly lower in XP group(P＜0.05);compared with the control group,the Myc group showed a significant increase in the relative abundance of Fibrobacter(P＜0.05).In summary,Myc reduces gas produc-tion for 48 h,which may inhibit rumen fermentation,but has no significant effect on methane emissions.XP can promote the production of butyric acid,improve the rumen environment and has a tendency to reduce methane emissions.Act has methane inhibition effect,which may change rumen fermentation mode by regula-ting rumen bacteria abundance,reduce acetate production in rumen and improve rumen environment to improve nutrient absorption efficiency and inhibit methane emission.[Chinese Journal of Animal Nutrition,2025,37(2):1059-1070]关键词
酵母培养物/奶牛/甲烷排放/体外发酵/瘤胃微生物Key words
yeast culture/dairy cows/methane emission/in vitro fermentation/rumen microbiota分类
农业科技引用本文复制引用
庄巧越,赵丽伟,雒诚龙,袁静,胡羽飏,王中汉,王倩倩,王蔚..酵母培养物对奶牛体外瘤胃甲烷排放、发酵参数及微生物区系的影响[J].动物营养学报,2025,37(2):1059-1070,12.基金项目
国家重点研发计划(2022YFD1301001) （2022YFD1301001）
财政部和农业农村部-国家现代农业产业技术体系(CARS-36) （CARS-36）
