动物营养学报2025，Vol.37Issue(2)：1162-1172,11.DOI:10.12418/CJAN2025.101
稻蛙种养模式下放养密度对黑斑蛙生长性能、血清激素和抗氧化指标及肠道菌群的影响
Effects of Stocking Density on Growth Performance,Serum Hormones and Antioxidant Indicators,and Intestinal Flora of Dark-Spotted Frogs in Rice-Frog Coculture System
摘要
Abstract
This experiment aimed to study the effects of stocking density on the growth performance,serum hormones and antioxidant indicators,and intestinal flora of dark-spotted frogs(Pelophylax nigromaculatus)in rice-frog coculture system.Dark-spotted froglets with initial body weight of(8.12±0.03)g were cultured at five different stocking densities of 6,12,24,48 and 96 frogs per square meter,further referred to as Ml,M2,M3,M4 and M5 groups,with three replicates in each group,and the frogs were cultured for 80 days.The results showed as follows:1)the survival rate of dark-spotted frogs significantly decreased with the stock-ing density exceeded 24 frogs per square meter.The average final weight,weight gain rate,and specific growth rate of dark-spotted frogs in group M3 were the highest,and the feed conversion ratio was significantly lower than that in groups M4 and M5(P＜0.05).2)There were no significant differences in serum cortisol,insulin-like growth factor-1,thyroid hormone contents,as well as superoxide dismutase,glutathione peroxi-dase,catalase activities and malondialdehyde content among the groups(P＞0.05),but the serum total antioxi-dant capacity in group M3 was significantly higher than that in group M5(P＜0.05).3)With the increase of stocking density of dark-spotted frogs,there was a trend of increase in the Chao1,Faith_pd,Observed_spe-cies,and Shannon's indexes of the intestinal flora,which were significantly higher in M5 group than that in M1 group(P＜0.05).4)At the phylum level,the relative abundance of Firmicutes in the intestinal flora in the M4 group decreased significantly(P＜0.05),and the relative abundance of Bacteroidetes in the M4 group in-creased significantly(P＜0.05)compared with the M1 and M2 groups.At the genus level,with the increase of stocking density of dark-spotted frogs,the relative abundance of Bacteroides in the intestinal flora showed an increasing trend,the relative abundance of Lactococcus showed a decreasing trend,and the relative abundance of Romboutsia and Akkermansia showed a trend of increasing and then decreasing.In conclusion,under the conditions of this experiment,too high stocking density of dark-spotted frogs is unfavorable to individual growth,and an appropriate increase in stocking density can improve the diversity of intestinal flora.It is recom-mended that the stocking density of dark-spotted frogs should be 12 to 24 frogs per square meter in the rice-frog coculture system.[Chinese Journal of Animal Nutrition,2025,37(2):1162-1172]关键词
黑斑蛙/放养密度/血清激素/抗氧化指标/肠道菌群Key words
Pelophylax nigromaculatus/stocking density/serum hormones/antioxidant indicators/intestinal flora分类
农业科技引用本文复制引用
韩光明,覃宝利,吴雷明,张家宏,寇祥明,王守红,徐荣,马林杰..稻蛙种养模式下放养密度对黑斑蛙生长性能、血清激素和抗氧化指标及肠道菌群的影响[J].动物营养学报,2025,37(2):1162-1172,11.基金项目
江苏省农业科技自主创新资金[CX(22)3184] （22）
扬州市生态农业重点实验室(YZ2023244) （YZ2023244）
